WESTERN BLOTTING ANALYSIS OF PROTEIN ANTIGENS OF FUSARIUM SOLANI

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AGHA MIRAN M.R.; ZEYNI F.

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Journal: JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES Year:2007 | Volume:15 | Issue:3 Page(s): 28-32

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Title

WESTERN BLOTTING ANALYSIS OF PROTEIN ANTIGENS OF FUSARIUM SOLANI

Author(s)

AGHA MIRAN M.R. | ZEYNI F. | Issue Writer Certificate

Keywords

FUSARIUM SOLANIQ3

WESTERN BLOTTINGQ3

ANTIGENQ3

Abstract

Introduction: WESTERN BLOTTING pattern of somatic protein ANTIGENs extracted from fungi can be useful in identification of different species and strains, and also helps to find the infectious agent. This study was undertaken to determine the somatic protein ANTIGENs pattern extracted from FUSARIUM SOLANI.Materials& Methods: The Fusarium Soani strains UAMH 7419 and UAMH 3317 used in our study to prepare the somatic protein ANTIGENs extracts from young mycelia were originally isolated from a patient and the environment in Canada, respectively. Determination of numbers and the protein ANTIGEN characteristics was carried out using sodium dodecy sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and WESTERN BLOTTING technique.Findings: Analysis of WESTERN BLOTTING patterns of somatic proteins from F. Solani UAMH 7419 and UAMH 3317 demonstrated 16 and 11 ANTIGEN bands, respectively. The molecular weights of protein ANTIGENs were between 14 to 100KD.Conclusion: The F. Solani strain UAMH 7419, isolated from the patient, showed more ANTIGENic bands in WESTERN BLOTTING than the F. Solani strain UAMH 3317 isolated from the environment.

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References

IMMUNOBLOTTING ANALYSIS OF SERA FROM PATIENTS WITH ACUTE AND CHRONIC VAGINITIS FOR IGE AND IGG ANTIBIODIES AGAINST CANDIDA ALBICANS

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APA: Copy

AGHA MIRAN, M.R., & ZEYNI, F.. (2007). WESTERN BLOTTING ANALYSIS OF PROTEIN ANTIGENS OF FUSARIUM SOLANI. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 15(3), 28-32. SID. https://sid.ir/paper/89783/en

Vancouver: Copy

AGHA MIRAN M.R., ZEYNI F.. WESTERN BLOTTING ANALYSIS OF PROTEIN ANTIGENS OF FUSARIUM SOLANI. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2007;15(3):28-32. Available from: https://sid.ir/paper/89783/en

IEEE: Copy

M.R. AGHA MIRAN, and F. ZEYNI, “WESTERN BLOTTING ANALYSIS OF PROTEIN ANTIGENS OF FUSARIUM SOLANI,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 3, pp. 28–32, 2007, [Online]. Available: https://sid.ir/paper/89783/en

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