PRODUCTION OF VITAMIN B12 AND LABELED VITAMIN B12 BY STREPTOMYCES GRISEUS

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MAZAHERI TEHRANI M.; GHORBAMZADEH MASHKANI S.; TAJER MOHAMMAD GHAZVINI P.; NAZARI AMIN; AFLAKI F.

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Journal: JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY Year:2009 | Volume:- | Issue:3 (49) Page(s): 43-49

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Title

PRODUCTION OF VITAMIN B12 AND LABELED VITAMIN B12 BY STREPTOMYCES GRISEUS

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Keywords

MICROBIAL FERMENTATIONQ3

STRPTOMYCES GRISEUSQ3

LABELED VITAMIN B12Q3

COBALT-57Q3

VITAMIN B12Q3

GAMMA SPECTROSCOPYQ3

Abstract

In this study, the production of LABELED VITAMIN B12 with one strain of actinomycet in a novel synthetic medium by batch fermentation method was investigated. After releasing vitamin B12 from microbial cells, the solution containing cobalamin analogues was fixed on amberlite XAD-4 exchanger and eluted by various solutions. Vitamin B12 in the collected fractions was measured by HPLC method and also in order to confirm the existence of cobalamin compounds in the samples, TLC technique was used. In the optimal conditions, production of vitamin B12 by this microorganism was 1456mg/l. The best recoveries of vitamin B12 from the column were obtained when the column was eluted by ethanol followed by acetone. The Rf values for cyanocobalamin and methylcobalamin, produced by this microorganism were the same as the standard values of Rf. The desorption of LABELED VITAMIN B12 was carried out by different eluants, including acetone, ethanol (absolute), ethanol 25% and deionized water. The activity of LABELED VITAMIN B12 was 8.55, 6.90, 0.79, 5.75Bq/ml, respectively. Therefore, the total specific activity of 21.99Bq/ml was obtained for LABELED VITAMIN B12.

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APA: Copy

MAZAHERI TEHRANI, M., GHORBAMZADEH MASHKANI, S., TAJER MOHAMMAD GHAZVINI, P., NAZARI, AMIN, & AFLAKI, F.. (2009). PRODUCTION OF VITAMIN B₁₂ AND LABELED VITAMIN B₁₂ BY STREPTOMYCES GRISEUS. JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY, -(3 (49)), 43-49. SID. https://sid.ir/paper/97695/en

Vancouver: Copy

MAZAHERI TEHRANI M., GHORBAMZADEH MASHKANI S., TAJER MOHAMMAD GHAZVINI P., NAZARI AMIN, AFLAKI F.. PRODUCTION OF VITAMIN B₁₂ AND LABELED VITAMIN B₁₂ BY STREPTOMYCES GRISEUS. JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY[Internet]. 2009;-(3 (49)):43-49. Available from: https://sid.ir/paper/97695/en

IEEE: Copy

M. MAZAHERI TEHRANI, S. GHORBAMZADEH MASHKANI, P. TAJER MOHAMMAD GHAZVINI, AMIN NAZARI, and F. AFLAKI, “PRODUCTION OF VITAMIN B₁₂ AND LABELED VITAMIN B₁₂ BY STREPTOMYCES GRISEUS,” JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY, vol. -, no. 3 (49), pp. 43–49, 2009, [Online]. Available: https://sid.ir/paper/97695/en

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