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Information Journal Paper

Title

INDUCTION OF MEIOTIC GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) WITH EMPHASIS ON TIME DETERMINATION OF SECOND POLAR BODY EXTRUSION

Pages

  120-127

Abstract

 In order to induced MEIOTIC GYNOGENESIS in rainbow trout (Oncorhynchus mykiss), spermatozoa genome was destroyed by DV IRRADIATION (10050 mw/m2) and after fertilization with intact ovum, diploidy was returned by thermal shock (28±0.5°c) in 15 minutes. Thermal shocks were applied in 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 minutes after fertilization. DV IRRADIATION was supplied from a germicidal lamp (15 w) with maximum intensity at 254 nm. Before irradiation, sperm was diluted with two kinds of solution and then irradiation, was done at 10 minutes on magnet stirrer. Albinisms were used as a phenotypic marker for monitoring of gynogenetic rainbow trout offspring. Furthermore, determination of diploidy was confirmed by preparation of chromosome slides from larval stages of fishes. Results show that, best gynogenetic yield was achieved in treatment which using shock on 35 and 50 minutes after fertilization.

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    Cite

    APA: Copy

    ESMAEILI, A.H., KALBASI, M.R., & POUSTI, I.. (2006). INDUCTION OF MEIOTIC GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) WITH EMPHASIS ON TIME DETERMINATION OF SECOND POLAR BODY EXTRUSION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 13(4), 120-127. SID. https://sid.ir/paper/9801/en

    Vancouver: Copy

    ESMAEILI A.H., KALBASI M.R., POUSTI I.. INDUCTION OF MEIOTIC GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) WITH EMPHASIS ON TIME DETERMINATION OF SECOND POLAR BODY EXTRUSION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2006;13(4):120-127. Available from: https://sid.ir/paper/9801/en

    IEEE: Copy

    A.H. ESMAEILI, M.R. KALBASI, and I. POUSTI, “INDUCTION OF MEIOTIC GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) WITH EMPHASIS ON TIME DETERMINATION OF SECOND POLAR BODY EXTRUSION,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 13, no. 4, pp. 120–127, 2006, [Online]. Available: https://sid.ir/paper/9801/en

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