Bioethanol production from starch by saccharomyces cerevisiae assessed with Response Surface Methodology (RSM)

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KAZEMI ALI; Shokri Mahsa; Mohammadi Mosa Alreza

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Paper Information

Journal: JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY Year:2020 | Volume:6 | Issue:3 Page(s): 29-35

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Title

Bioethanol production from starch by saccharomyces cerevisiae assessed with Response Surface Methodology (RSM)

Author(s)

KAZEMI ALI | Shokri Mahsa | Mohammadi Mosa Alreza | Issue Writer Certificate

Keywords

Bioethanol

Response surface methodology

Saccharomyces cerevisiae

Starch

Abstract

Saccharomyces cerevisiae has many different uses such as baker's yeast and bioethanol production. Response surface methodology (RSM) is a complex of statistical techniques for experimental model creation. In this study was done to determination of optimal condition of bioethanol production from starch by yeast using RSM. Initially saccharomyces cerevisiae was proliferated on agar then by α-amylase, dextranase and amyloglucosidase respectively, then starch 5% was hydrolyzed to glucose. 250 ml glucose was put at 30 º C and velocity of 150 rpm for 36 h. Bioethanol quantity was evaluated by dichromate oxidation and ethanol measurement methods and temperature, time and pH variables were considered at 25-98 º C, 1 to 6 weeks and 3 to7, respectively. The highest bioethanol production was 12. 5 ml at 30 º C, pH 4 also at 80 º C, and pH 7 for 5 weeks. The lowest bioethanol production was 3 ml at 98 º C, pH 7 for 1 week which was practically compatible. Regarding the results, RSM method has many benefits such as increase in efficiency of chemical reactions, prevention of wasting raw material, time, money and energy which could use in different biological reactions.

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APA: Copy

KAZEMI, ALI, Shokri, Mahsa, & Mohammadi, Mosa Alreza. (2020). Bioethanol production from starch by saccharomyces cerevisiae assessed with Response Surface Methodology (RSM). JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, 6(3 ), 29-35. SID. https://sid.ir/paper/985295/en

Vancouver: Copy

KAZEMI ALI, Shokri Mahsa, Mohammadi Mosa Alreza. Bioethanol production from starch by saccharomyces cerevisiae assessed with Response Surface Methodology (RSM). JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY[Internet]. 2020;6(3 ):29-35. Available from: https://sid.ir/paper/985295/en

IEEE: Copy

ALI KAZEMI, Mahsa Shokri, and Mosa Alreza Mohammadi, “Bioethanol production from starch by saccharomyces cerevisiae assessed with Response Surface Methodology (RSM),” JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, vol. 6, no. 3 , pp. 29–35, 2020, [Online]. Available: https://sid.ir/paper/985295/en

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