Effects of six levels of nitrogenous fertilizers (urea) on yield and total alkaloids content in different Organs of Physalis alkekengi were investigated. All experiments were performed at Medicinal Plants Research Station a branch of Yazd Province Research Center for Natural Resources and Animal Husbandry.Experiments were designed as a completely randomized blocks. Six levels of urea fertilizer namely, 0, 50, 100, 150, 200 and 250 kg/ha with three replicates were applied to the soil.Upto a certain level, there was a positive correlation between the amount of Napplied and the plants yield (leaves, stems and roots). The correlation between urea application and plants canopy and height was also positive.Whereas the highest plant yield was obtained with 150 kg urea/ha the maximum plant height was achieved with the application of 100 kg urea/ha. Any increase in the amount of N-fertilizer beyond 150 kg/ha had an adverse effect on plant yields.The total alkaloids content in leaves, fruits and roots were determined by yanevan method.Increasing the amount of urea resulted in an increase in total alkaloid content of leaves, fruits and roots.Application of urea at a rate of 100, 150 and 200 kg/ha caused the highest increase in total alkaloids. Since there was no significant difference (a= 0.01) among the means of alkaloids produced in these treatments, it was concluded that the highest alkaloids will be obtained by application of urea at a rate of 150 kg/ha.Increasing the amount of urea did not affect the rate of root expansion significantly. However, root depth decreased in response to urea application. The highest root depth was obtained in control (no urea) plants. This is in agreement that N-Fertilizers will mostly increase the amount of shoot rather than root system.