Background: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis.Objectives: Development of the drug resistance is becoming a threat to disease control, which underscores need for new agents targetingM. tuberculosis.Materials and Methods: In this study, analysis of gene expression was performed using real-time polymerase chain reaction (RTPCR).Results: Results of the current study showed that the minimum inhibitory concentration value of cinnamaldehyde against M. tuberculosis was 200mg/mL. Moreover, RT-PCR data showed that a total of 25 genes were regulated by the cinnamaldehyde. Of these, 12 genes were up-regulated, and 13 genes were down-regulated.Conclusions: Cinnamaldehyde is a pattern to expand the new anti-TB drugs, because the targets of the cinnamaldehyde are different from those of anti-tubercular agents.

Background & Aims of the Study: Foodborne illnesses, resulting from consumption of contaminated foods, represent a substantial public health threat. Listeria monocytogenes is a foodborne pathogen bacteria and can cause serious problems in human. Because of this reason, Nowadays various compounds are being used to control these bacteria in foods. The aim of this study was to evaluate antilistarial potential of barberry extract, cinnamaldehyde (CA) and nisin, also to discover synergistic effects of dual combination of these compounds againt Listeria monocytogenes in culture medium. Material and methods: The antilisterial activity of barberry extract, cinnamaldehyde (CA) and nisin was evaluated using agar well diffusion method and minimal inhibitory concentration (MIC), also Combined antilisterial activity was examined by fractional inhibitory concentration (FIC) and time-kill assays. Results: In agar well diffusion method, nisin (400 IU/ml) and CA (40 μ l/ml) produced the largest inhibition zones (20. 75± 0. 25 and 32. 0± 1, respectively). MIC values of nisin, CA and barberry extract were 25 IU/ml, 0. 312 μ l/ml and 37. 5 mg/ml, respectively. In time kill assay, the combination of nisin with CA in MIC concentration was found the best combination against L. monocytogenes and a 6 log reduction in bacterial count was obtained. Conclusion: The results of this study revealed that nisin and CA have convenient antilisterial activity and combination of these two compounds in MIC concentration showed synergistic effects against Listeria monocytogenes.

In the present investigation, HOMO-LUMO energy gap, polarizability, structural parameters, molecular mass, hardness, chemical potential and electrophilicity index of the trans-cinnamaldehyde as one of the active ingredients of cinnamon in the gas phase and in ethanol, acetone and dichloromethane solvents were calculated. Density Functional Theory calculations were performed by the B3LYP method with 6-311G+(d,p) and DGDZVP basis sets on the compounds. According to the calculations, HOMO-LUMO energy gap and hardness in the gas phase was the largest and in ethanol was the smallest, polarizability, softness, dipole moment, electrophilicity had the largest amount in ethanol and the smallest in the gas phase, chemical potential of compound in dichloromethane was the greatest and in the gas was the lowest.