Background: Among different kinds of cells involved in the wound healing process, fibroblasts play a pivotal role in the proliferation phase of this procedure wherein they induce the production of local growth factors and cytokines. Fetal fibroblasts with low immunogenic property and different wound healing process could be considered as a suitable alternative to neonatal foreskin cell based products in regenerative medicine and cell therapy. Cell therapy is an almost newly introduced method in the medical field and is also a challenging issue, which should assure the safety of the final product and the absence of viral or bacterial contamination with minimal immunogenic response. Therefore, based on current GMP (cGMP) principles, it is a matter of great importance to monitor raw materials’ quality and the conformity of all production procedures including cell culture, collection, and cryopreservation with validated standard operating procedures (SOPs). In the current study, we demonstrated the GMP-compatible and clinical-grade fetal fibroblast cells banking to be used for clinical applications. Methods: All steps of isolation and culturing and cryopreservation of fetal fibroblast cells were performed under the sterile condition according to cGMP guidelines in the clean room. During the cell culture procedures, bacteriological investigation was performed in order to identify the probable contamination of the samples. Results: Isolated fetal dermal fibroblasts were harvested and stored at early passages. The viability of fibroblasts was 98%, indicating successful establishment of fibroblast banking. Flow cytometry analysis has revealed the high purity of isolated and cultured fibroblastic population Karyotyping of fibroblasts at the 10th subculture shows a normal diploid male pattern. Conclusions: The manufactured cells, according to the highest level of safety are considered as the most appropriate treatment option for cellular therapy. Based-on the current evidence, clinical-grade fetal fibroblasts throw new light on efficient and prompt healing of acute and chronic wounds of any etiology.

The high molecular weight polymeric fraction of gluten, glutenin macro-polymer (GMP) shows strong relation with breadmaking quality of wheat. In this study, GMP was extracted from different Iranian wheat cultivars with different breadmaking performances. The correlation was investigated between the wet weight and (small-strain deformation) rheological characteristic of GMP-gel with bread quality (loaf volume and height). The results showed a significant relation between gel amount and bread loaf volume and height. However, no positive and significant correlation was found between small-strain deformation rheological characteristics (storage modulus and tan delta) with breadmaking characteristics. Concluding, despite to GMP wet weight which is accepted as a predictive measure for breadmaking quality, small-strain deformation rheological analysis of GMP-gel can not be used for screening breadmaking quality between wheat cultivars.

In order to study of cause and effect relationships of agronomic characters in water stress and water non-stress conditions with Geometric Mean Productivity index (GMP), a research was conducted in experimental field of agriculture faculty of Zanjan University in the year 2003. In use Experimental design was split block design based RCBD with three replications that irrigation with two level (water non-stress and water stress) and rapeseed variety in sixteen level were experimental factors. The results of sequential path analysis indicated that for selecting under water non-stress conditions in order to breeding for grain yield GMP, essentially was after increasing number of branches and siliqua per plant and decrease of 1000-grain weight. Due to relationship between these three characters is necessary to put them in equilibrium together. Also for selecting under water stress conditions essentially was after increasing of seeds per siliqua and siliqua per plant and decrease of days to flowering. Indirect breeding for oil yield GMP in water non-stress conditions is possible by breeding to increase of number of branches and siliqua per plant and to decrease of 1000-grain weight and under water stress conditions by breeding to increase of seeds per siliqua, siliqua per plant and oil content.

Background and Objectives: Pre-requisite programs (PRPs) are “primary conditions and requirements essential for HACCP operations, which are crucial in food safety programs”. The present study was conducted to evaluate the impact of implementation of PRPs on the microbial parameters of pasteurized milk (according to the National Standard of Iran). Effectiveness of HACCP operation requirements and efficiency of Good Laboratory Practice (GLP) were also evaluated in control of the above-mentioned microbial parameters.Materials and Methods: According to the approved checklist of the Vice-chancellor in Food and Drug affairs, PRPs of 26 factories were evaluated from March 2014 to March 2015 in two-month intervals, and their total and component scores were obtained along with the microbial parameters of pasteurized milk. Generalized Estimating Equations (GEEs) were used to determine the significance of total score and the impact of its components on controlling microbial hazards.Results: There was a reverse significant relation between the total scores of the PRPs and microbial hygiene indices (total and coliform count) which approves the effectiveness of operating the programs in controlling the mentioned microorganisms. Efficiency of each pre-requisite program was different in controlling the microbial parameters. Good Laboratory Practice (GLP) had a prominent effect on controlling of the index microorganisms of hygienic operations. Overall, the results showed a little probability of contamination with E.coliin the pasteurized milk samples of Fars Province for which the statistical analysis was ignored.Conclusions: The exact operation of PRPs resulted in reduction of microbial parameters in a way that increasing the total score of PRPs led to decrease in microbial parameters of total count (TC), coliforms, molds and yeasts. The findings further suggest the application of this checklist in evaluation and prediction of microbial parameters.