Rapid growth, feed efficiency, growth performance and disease resistance are the most important concerns in industrial aquaculture. In some food ingredients, the role of gut flora in feed efficiency and host health has been shown. Different approaches to study intestinal microflora have been used to improve growth performance, nutrient digestibility, and stimulation of the immune response, survival and resistance in many animals. Prebiotics, classified as a non-digestible food ingredient, beneficially affects the host by selectively stimulating the growth, activating a limited number of bacteria, and reducing the number of harmful bacteria in the gastrointestinal tract. Such information on prebiotics in aquatic organism is still inadequate.