The moderating role of salicylic acid (SA) and putrescine (PUS) as plant growth regulators (PGRs), on the growth parameters and phytochemical and qualitative characteristics of strawberry fruit 'Selva' under osmotic stress was investigated under soilless culture. The osmotic potential (salinity) of the nutrient solution containing different NaCl concentrations (0, 7. 5, 15, 30 and 45 mM) and foliar application of PUS (0 and 1. 5 mM) and SA (0 and 1. 5 mM) were studied. The results showed a significant decrease in plant leaf area (79. 6%), total chlorophyll content (48%), fruit yield (73. 5%), leaf relative water content (33%), total protein (33. 4%), total phenol (7. 8%), and vitamin C content (24. 5%) under osmotic stress. Moreover, peroxidase (POD) and superoxide dismutase (SOD) enzymes activity, leaf ion leakage, and soluble carbohydrate and proline content increased significantly under osmotic stress. Application of PGRs had a significant effect on all the studied traits (except for SOD activity). Interactive effects of salinity and PGRs were significant on all the traits except for leaf ion leakage, POD activity, soluble carbohydrates, and protein. The highest total phenol and vitamin C contents were obtained with 15 mM salinity along with foliar application of PGRs. In conclusion, foliar application of PUS and SA ameliorate negative effects of salt stress on growth, yield, and quality of strawberry fruit.