The amount of cosmic rays varies widely with the altitude, latitude and longitude in each region. In this study, the radiation doses due to the cosmic rays were estimated in two steps: in the first step, the neutron and gamma components of the radiation dose were measured for a roundtrip flight on 3 flight routes (Shiraz-Asaluye, Asaluye-Rasht and Shiraz-Mashhad) using a gamma-tracer photon detector and a Thyac 190N, neutron detector. The minimum values of the measured gamma and neutron doses of 0.15 and 0.04 mSv were measured on the Asaluyeh-Shiraz route at the lowest altitude of 19000 ft, while for Rasht-Asaluyeh route at an altitude of 35000ft those values were found to be 2.52 and 1.09mSv, respectively. In the second step, a number of aircrew members were equipped with thermoluminescence dosimeters (TLD cards) for evaluating the gamma dose and polycarbonate dosimeters (SSNTD) for assessing the neutron dose for one year. The measured value of the annual effective dose received by the crew ranged between 0.5 mSv/y and 1.16 mSv/y, with an average of 0.9 mSv/y for the gamma component and between 0.37 mSv/y and 0.77 mSv/y with an average of 0.61 mSv/y for the neutron component. The results of this investigation are comparable with the investigations that have been conducted in other countries. For instance in UK, the reported annual effective dose of aircrew is about 2mSv, and in Canada, it is estimated to be between 1 to 5mSv, depending on the flight situations (such as the latitude and longitude of the cities, the flight altitude, etc).