Introduction: The purpose of the present study was to evaluate the effect of six- week aerobic step training on cardiovascular fitness, body composition, flexibility, leg anaerobic power and quality of life of Isfahan University female students in academic year of 2011-2012.Materials and Methods: Thirty-one female students of Isfahan University were randomly assigned to experimental group (N=16, mean age of 27±2, weight 58.34±6.27, height 1.61±4) and control group (N=15, mean age of 25 ±1, weight 54.7±7.32, height 1.61±6). Pre-test and post-test measurements included cardiovascular fitness, body composition, flexibility, leg anaerobic power and quality of life questionnaire (WHOQOL). Analysis of covariance (ANCOVA) was used to evaluate to effects of aerobic training as intervention (P<0.05).Results: There was a significant increase in cardiovascular fitness (p=0.001) and there was a significant decrease in body composition (p=0.046). There was no significant increase in flexibility (p=0.109) and leg anaerobic power (p=0.180). There was a significant increase in total score of quality of life (p=0.00).Conclusion: A six-week aerobic step training improved quality of life, cardiovascular fitness and body composition.