Rosa damascena Mill. Genotypes from different areas of Iran were cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. 11 genotypes were compared based on flower yield and morphological characteristics, including plant height, canopy, spine density, spine length, branch angel and leaf number. Evaluation of the obtained data was performed using ANOVA, mean classification and correlation analysis. Genotypes showed significant differences in terms of the measured traits. Comparison of means, classified the genotypes in 3, 4 and 3 groups, based on flower yield, flower number, and average flower weight, respectively. Also, the genotypes were classified in different groups, based on morphological characteristics. The results also indicated that there is a good relationship between some of the measured traits, particularly between yield and number of flowers. It could be concluded from the results of different analyses that although the genotypes could be grouped based on geographical origins, flower yield and number and some of the morphological characteristics could be used as appropriate selection factors for classification of damask rose genotypes, in order to use in breeding programs.