In order to investigate the effect of planting date and various levels of the nitrogenous fertilizer on the effective substances, biological yield and production of chamomile, an experiment was conducted during 2004-2005 on the research farm of faculty of agriculture of Islamic Azad University Khorasgan Branch. In this experiment three planting dates (6 March, 20 March and 5 April) and three levels of nitrogenous fertilizer (75, 150 and 225 kg per hectare) were evaluated as Urea fertilizer. The number of flower, dry and wet weight of flower in three cuttings, the number of days to budding, the number of days to 50% flowering, the number of days to 100% flowering, the height and the diameter of stems, and the number of branches, tillers, and the percentage of essence and the weight of essence were measured. The results showed that the various planting dates with regard to the number of total number of flowers, the total wet weight, the total dry weight, the numbers of days to flowering, the number of days to 50% flowering, the number of days to 100% flowering, the height of stem and the diameter of stem had a significant difference at one percent probability and the percentage of essence had a significant difference at five percent probability. On the first planting date, the effect of the earlier planting dates on the number of days to the budding date was two weeks longer. The delay in the planting date had no significant effect on the stems, tillers, branches and percentage of essence and the weight of essence. The effect of the level of nitrogen on the number of days to 50 percent of flowering and the number of branches were significant at 5 percent probability, and the rest of the characters were not affected by the level of nitrogen in the soil. There was a significant interaction between the planting date and the level of nitrogen on the number of flowers in the first cutting, wet weight of flowers and the percentage of essence. There was a significant interaction between planting date and the level of nitrogen on the percentage of essence. The highest number of flowers were obtained on the first planting date using more than 75 kg nitrogenous fertilizer per hectare. The best percentage of the essence was related to the third planting date and 150 kg nitrogenous fertilizer per hectare. It is expected that the greatest rate of treatment in chamomile is obtained by planting the crop on the first of March, according to the earlier planting dates and the use of nitrogenous fertilizer, lower than 75 kg per hectare.