Introduction: Netiquette is network etiquette or the etiquette of cyberspace. Recognizing organizational culture and its relationship with the netiquette offers strategies applied in order to communication development and creating learning organizations through the internet for senior management. The purpose of this study was to determine the relationship between organizational culture and netiquette among academic staff in IUMS.Methods: In this correlation descriptive study, 138 out of 594 faculty members of IUMS was selected randomly using simple stratified sampling method, in 2008. Data collected using two; Organizational Culture's questionnaire and Netiquette Culture's questionnaire with 97% and 89% reliability. Ultimately, SPSS15 was used for the statistical analysis of the data.Results: There was no significant relationship between netiquette and organizational culture of the faculty members. As an illustration, sample members' favoring the team, focusing on details, seeking stability and courage outcomes varied in terms of their sex. In addition, regarding achievements and courage varied significantly concerning their employment record. Finally, members' viewpoint about innovation, favoring the team, risk taking, focusing on details, seeking stability, and courage outcomes was different significantly based on their educational certificate and academic rank. There was no significant relationship between netiquette and demographic variables.Conclusion: Overall, organizationally culture and netiquette can be improved by engaging members in activities requiring cooperative thinking and exchange of knowledge; for the promotion of netiquette, the lows of netiquette must be taught, as well as, facility communication among members base on custom and traditions which must be train in Isfahan Medical university and in other government offices trough out the country.