Cytological information is absolutely lacking for the Iranian moss flora (Bryophytes). The present research, dealing with cytological studies on the following five species (including two expressions and one variety) of Iranian mosses, namely, Amblystegium riparium (Hedw.) B.S.G. "brevipes" expression (n=20), A. riparium (Hedw.) B.S.G. ''pennellii'' expression (n=9, 9+m, 9+2m), A. serpens (Hedw.) B.S.G. (n=20), Campylium stellatum (Hedw.) C. Jens. in Lange var. protensum (Brid.) Bryhn ex Grout (n=10), Fissidens taxifolius Hedw. (n=12+m) and Orthothecium intricatum (Hartm.) Schimp. in B.S.G. (n=11) is performed for the first time in Iran. The first three species (plus two expressions and one variety) belong to the order Hypnales and the family Amblystegiaceae. O. intricatum is placed in the said order but the family Entodontaceae (according to some authors it is considered under a different family of the same order i.e. Hypnaceae), and lastly, F. taxifolius included in the order Fissidentales and the family Fissidentaceae.In the present study, fertile moss specimens were collected from the northern provinces of the country during spring and summer. Capsules with very light brown opercula were fixed in 1:3 acetic alcohol for about 24 hrs. and meiotic chromosomes were studied by squashing (tapping) and heating the sporogenous tissue in 2% acetocarmine. Prepared slides were observed under the microscope to count the chromosome numbers and then photomicrographs were taken from each slide.