An experimental investigation has been done on the effective parameters of biogas production from the mixture of food waste (FW), cow manure and organic additives. The samples of FW were collected from the university restaurant and they have mixed with the cow manure (CM) with different ratios of (FW/CM) 1: 1, 1: 2, 1: 3, and 3: 1 in a one liter polymeric bottle. These bottles embedded in a hot water bath at a thermophilic temperature of 55-60 ° C in the period of 15 days and the volume of produced biogas was measured. The results were shown that with the ratio of 1: 1 the highest volume of gas was collected (5 liters). Then 10 grams of chemical additive of silica gel and organic additives like rosemary plant, walnut shells, and banana and tomato skin were added to the optimum selected ratio of digester. The produced biogas was increased 24. 4%, 7. 2%, 2. 3%, 1. 9% and 6. 6% by volume respectively in compare with that of optimum based mixture. During the digestion process, it was found the highest volume of biogas was collected when the pH of the mixture was in neutral range (7) and the use of walnut shells resulted to pH stabilization in the process. Measuring physical properties (total solids, volatile matter, moisture content, and ash content), for the mixture of food waste and manure were shown the high capacity of food waste to convert to biogas based on the laboratory experiments.