Introduction: One of the most important indicators in relation to industry, economy and environment is to achieve the most recovery with the least cost and minimum pollution. Today, the use of chemolithotrophic microorganisms is common for extraction of some metals such as cooper, uranium, gold, cobalt and molybdenum in bioleaching process of low-grade rocks. Attention to the bioleaching of heavy metals such as copper, nickel, cobalt, zinc, and molybdenum has been increased in recent years because of its application to low-grade ores. …