Considering the environmental effects of using pesticides and the gradual decrease in the amount of crops, the potential of allelopathic plant extracts can be used for biological controlling and releasing the allelochemical substances from leaves, seeds, roots, and stems of the living plants or plant substances that are getting decomposed. This study was carried out under the laboratory and greenhouse conditions, in an attempt to investigate the effect of hydro-alcoholic extracts obtained from the roots and shoots of annual wormwood (Artemisia annua L.) medicinal plant on the germination and growth of four types of weeds including Avena fatua L., Chenopodium album L., Setaria viridis L., and Amaranthus retroflexus L. The treatments included the extracts of annual wormwood in six concentrations (0.1, 0.25, 0.5, 1, 2.5 and 5%) and the distilled water as the control. The results indicated that different concentrations of the extracts of annual wormwood significantly decrease the germination percentage as well as the length of radicle and plumule in weed seeds under the laboratory condition. In addition, different concentrations of the extract resulted in a significant decrease in the growth percentage of the seeds, the height, and wet and dry weights of weed bushes under the greenhouse condition. The laboratory results revealed that the seed germination in Avena fatua L., Chenopodium album L., Setaria viridis L., and Amaranthus retroflexus L. stopped using extracts with concentrations of 0.25, 0.5, 0.5, and more than 2.5% respectively. The greenhouse results indicated that the annual wormwood extract with 1% concentration respectively reduces the germination percentage of the seeds of Avena fatua L., Chenopodium album L., Setaria viridis L., and Amaranthus retroflexus L. to 64, 84, 43, and 65% in comparison to the control. It was also found that a 5% concentration of the extract nearly stopped the seed germination in Avena fatua L. and Setaria viridis L. On the basis of the results, it can be claimed that the extracts of the annual wormwood have strong allelopathic effects and inhibit germination and growth of the weeds, a quality which can be applied in producing natural pesticides.