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Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Title: 
Author(s): 

Issue Info: 
  • Year: 

    0
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    -
Measures: 
  • Citations: 

    0
  • Views: 

    841
  • Downloads: 

    0
Keywords: 
Abstract: 

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 841

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Title: 
Author(s): 

Issue Info: 
  • Year: 

    0
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    -
Measures: 
  • Citations: 

    0
  • Views: 

    2876
  • Downloads: 

    0
Keywords: 
Abstract: 

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 2876

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Issue Info: 
  • Year: 

    1389
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    37-41
Measures: 
  • Citations: 

    0
  • Views: 

    483
  • Downloads: 

    0
Abstract: 

برای مطالعه تاثیر لیگاتور دو طرفه شریان رحمی بر روی پراکندگی ماست سل ها 24 خرگوش ماده بالغ مورد استفاده قرار گرفتند. بعد از لیگاتور شریان رحمی در روزهای 23، 43 و 63 عمل جراحی برداشت تخمدان بر روی حیوانات صورت پذیرفت. نمونه های بافت شناسی برای بررسی پراکندگی ماست سل ها توسط روش تولوئیدن بلو رنگ آمیزی و مورد مطالعه قرار گرفتند. مشاهدات نشان دادند که ماست سل ها در گروه تست به تعداد زیاد در ناحیه ناف تخمدانی دیده می شوند. در ضمن این سلول ها به شکل گروهی در نزدیکی عروق خونی بخش های لوله ای دستگاه تناسلی مشاهده شدند. این در حالی است که این سلول ها در گروه کنترل پراکندگی متوسطی را در اطراف عروق خونی نشان دادند. مضافا اینکه پراکندگی این سلول ها در اطراف عروق خونی قسمتهای مختلف دستگاه تناسلی به شکل معنی داری (P=0.05) کمتر از گروه های تست بود. در نهایت اینگونه می توان نتیجه گیری کرد که با توجه به اینکه پراکندگی و نحوه قرارگیری ماست سل ها به شرایط فیزیولوژیک و پاتولوژیک عضو وابسته می باشد، لذا متعاقب لیگاتور دو طرفه شریان رحمی پراکندگی و گستره این سلول ها تغییر می کند.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 483

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Issue Info: 
  • Year: 

    1389
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    59-62
Measures: 
  • Citations: 

    0
  • Views: 

    504
  • Downloads: 

    0
Abstract: 

مجتمع سازگاری بافتی نقشی اساسی در کنترل مقاومت به بیماری و پاسخهای ایمنی دارد. تنوع ژنتیکی گسترده در ژن های مجتمع سازگاری بافتی سبب فراهم شدن یک منبع با ارزش برای بهبود ژنتیکی از طریق انتخاب در بسیاری از گونه های حیوانات اهلی می شود. از اگزون دو کلاس II مجتمع سازگاری بافتی در گوسفند اهلی (اویس آریس) می توان برای رسیدن به این هدف و مطالعات بعدی ژنتیک جمعیت استفاده کرد. در این مطالعه ژن Ovar-DRB1 در 138 نمونه DNA گوسفند ایرانی نژاد سنگسری با استفاده از روش PCR-RFLP مورد بررسی قرار گرفت. هشت آلل DRB1 در بین گوسفندان ایرانی نژاد سنگسری شناسایی گردید. یک آلل جدید نیز که قبلا شناسایی نشده بود در بین آلل های شناسایی شده این مطالعه مشاهده گردید. آنالیز PCR-RFLP باعث شناسایی سریع تایپ های Ovar-DRB1 و تفریق سریع بین ژنوتیپ های هموزیگوت و هتروزیگوت می گردد. نتایج مطالعه حاضر مشخص کرد که اگزون دو ناحیه ژن Ovar-DRB1 در نژاد سنگسری بسیار پلی مورف می باشد.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 504

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Issue Info: 
  • Year: 

    1389
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    49-52
Measures: 
  • Citations: 

    0
  • Views: 

    639
  • Downloads: 

    0
Abstract: 

لپتوسپیروز یک بیماری زئونوز (مشترک بین انسان و دام)، با گستردگی جهانی و مخازن متعدد است که در اثر عفونت ناشی از سرووارهای لپتوسپیرا اینتروگانس سنسو لاتو ایجاد می گردد. این مطالعه جهت تعیین میزان شیوع عفونت ناشی از لپتوسپیرا در گربه های ولگرد شهرستان اهواز، در جنوب غرب ایران و فاصله زمانی فروردین ماه 1386 لغایت خرداد ماه 1387 انجام شده بود. نمونه های خون از 102 قلاده گربه ولگرد جمع آوری شد. آنها به روش آگلوتیناسیون میکروسکوپی (MAT) مورد ارزیابی قرار گرفتند. از مجموع 102 قلاده گربه مورد بررسی، 5 مورد (4.9 درصد) از نظر سرولوژیکی بر علیه حداقل یک سرووار از لپتوسپیرا مثبت بودند. بیشترین تعداد از واکنش های مثبت بر علیه لپتوسپیرا اینتروگانس سرووار بالوم، در 5 نمونه سرمی بود. آنتی بادی بر علیه بیش از یک سرووار (بالوم و استرالیس) در یک نمونه دیده شد. تمام نمونه های مثبت تیتر 1:100 داشتند. شیوع عفونت در گربه های نر (5.26%) و ماده (4.444%) بودند. آنالیز آماری تفاوت معنی داری را بین جنس های مختلف نشان نداد، اما تفاوت بین گروههای سنی، معنی دار بود (0.021=P) چرا که تمام گربه های با تیتر مثبت، سن بالاتر از 3 سال را داشتند. این مطالعه اولین گزارش از سرووارهای بالوم و استرالیس در گربه های ایران است.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    1389
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    63-67
Measures: 
  • Citations: 

    0
  • Views: 

    1584
  • Downloads: 

    0
Abstract: 

بمنظور بررسی ترکیبات آغوز و استفاده از این ماده بعنوان مکمل غذائی برای اهداف مختلف مطالعه ای انجام شد. در این مطالعه اثر مکمل غذائی آغوز گاوی بر روی مالون دی آلدئید سرم(MDA) ، فعالیت آنتی اکسیدان (AOA) و گلوکز در مدل حیوانی جونده دیابتی بررسی شد. برای این مطالعه 60 رت نر Wistar انتخاب و به 10 گروه 6 تائی برای مدت 40 روز به ترتیب زیر تقسیم شدند: گروه غیر دیابتی، گروه دیابتی، گروه های دیابتی همراه با مصرف 10%، 20 % و 30% آغوز، گروه های غیر دیابتی همراه با مصرف 10%، 20% و 30% آغوز، گروه دیابتی همراه با مصرف انسولین، گروه دیابتی همراه با مصرف گلیبن کلامید. اگر چه سطح (MDA) سرم یک کاهش معنی داری در پاسخ به انسولین (2.56±0.31 µmol/dl) و مصرف 10%، 20% و 30% آغوز به ترتیب 0.46±0.04 µmol/dl) ، 0.29±0.06و 0.09±0.37) نشان داد اما کاهش در رت های دیابتی(3.92±0.29 µmol/dl) بیشتر بود (P<0.01) اختلاف معنی داری در AOA در هر دو گروه رت با مصرف انسولین (0.78±0.11 mmol/dl) و گلیبن کلامید (0.7±0.08 mmol/dl) در مقایسه با رت های دیابتی (0.69±0.1 mmol/dl) دیده شد; در هر حال AOA افزایش معنی داری در پاسخ به %10,(1.78±0.11 mmol/dl) (1.57±0.02 mmol/dl)20%و %30 (1.75±0.02 mmol/dl) مصرف آغوز نشان داد (P<0.001) همه گروه های درمانی یک کاهش معنی داری در میزان گلوکز سرم در مقایسه با گروه های دیابتی (391±79.39 mg/dl) نشان دادند(P<0.01). بنظر میرسد که آغوز یک مکمل مفید در رژیم غذائی در کاهش MDA سرم و سطح گلوکز و افزایش AOAسرم در دیابت ملیتوس تیپ I باشد.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    1-4
Measures: 
  • Citations: 

    0
  • Views: 

    1166
  • Downloads: 

    233
Abstract: 

Fungal agents, especially yeasts, are resident in digestive system as microflora and they can invade the mucosal tract in animals with predisposing factors. The prevalence of the resident fungal flora in the digestive tract of healthy broilers and layers during April to September 2008 was investigated. The samples were taken from the preventriculus, jejunum and cecum contents of 120 birds and cultured on Sabouraud glucose agar. Both filamentous fungus and yeasts were isolated and a predominance of the Candida species was found, followed by Trichosporon beigelii, Geotrichum candidum Rhodotorula rubra, and Saccharomyces cerevisiae species, which were found in 84.6%, 5.5%, 4.6%, 3.3% and 0.5% of cultures, respectively. Among the yeast isolates, Candida albicans (45.8%) was the most prevalent fungal agent, representing a significant proportion when compared to the other fungal isolates (P<0.05). The frequency of the yeast in the layers' digestive tract was significantly higher than that found in the broilers (P<0.05). Mycological examination revealed the highest numbers of yeasts in the cecum and lower numbers in the jejunum and preventriculus in two breeds.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    5-8
Measures: 
  • Citations: 

    0
  • Views: 

    920
  • Downloads: 

    200
Abstract: 

euthanatized and a complete necropsy was performed. Microscopic sections were prepared and stained with hematoxylin and eosin, Gram staining, periodic acid-Schiff (PAS) and acid-fast staining. Escherichia coli was isolated in pure culture. Necropsy revealed the presence of granulomatous lesions of varying sizes at different locations and hepatomegaly, oviduct impaction and oophoritis. Histopathologically, typical granuloma with a central area of coagulation necrosis and bacterial colonies surrounded by lymphocytes, macrophages and multinucleated giant cells were found. These granulomas were present in the liver, oviduct and intestinal tract. A sheet of neoplastic cells and disruption of the normal hepatic architecture was seen. The diagnosis was lymphoid leucosis and coligranuloma.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 920

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    9-12
Measures: 
  • Citations: 

    0
  • Views: 

    2515
  • Downloads: 

    240
Abstract: 

Cases of minimally-invasive surgery are gaining acceptance among surgeons and animal owners. One type of minimally-invasive surgery is total splenectomy, which is indicated for splenic tumors, trauma, torsions, and for dogs that require blood transfusion. To assess the safety of the technique, experimental laparoscopic splenectomies were performed in dogs for the first time in Iran. Three adult male mixed-breed dogs were placed in dorsal recumbency at a 45o angle in the right lateral position, under aseptic condition and general anesthesia. Three trocars were placed; at the umbilicus, 3 cm cranial to the umbilicus and in the right caudolateral abdomen. The spleen was rotated 90 ° laterally and thermal coagulation of the blood vessels was performed by bipolar electrocautery. The pedicles were checked to ensure hemostasis. The spleen was morcellated and removed from the umbilical port using a retrieval bag. The mean surgery time was 42 minutes, and all dogs survived the surgery without complications.Laparoscopic splenectomy via three portals was found to be easy and safe.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 2515

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    13-16
Measures: 
  • Citations: 

    0
  • Views: 

    1474
  • Downloads: 

    191
Abstract: 

The aim of this study was to determine the prevalence and type of ophthalmic diseases among horse populations in the suburbian riding clubs of Tehran. Ophthalmologic examinations were performed in 901 horses and ocular lesions were diagnosed in 40 (4.4%) animals. In the 54 eyes of these horses that had ocular defects, 103 ocular abnormalities were detected. Of those horses affected, 65% showed abnormalities in one eye and 35% had abnormalities in both eyes. Various sequelae to ocular trauma and periodic ophthalmia were the most common and recognizable causes, which comprised 25% and 15% of horses with ocular defects, respectively.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 1474

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    17-20
Measures: 
  • Citations: 

    0
  • Views: 

    2880
  • Downloads: 

    228
Abstract: 

Various forms of intensive sport place stress on the musculoskeletal system of the horse, during both racing and training. The musculoskeletal system of the horse has an inherent ability to adapt to the demands of high speed exercise, but exceeding the threshold of adaptive capacity may result in some form of damage. Continuing racing or training may delay the repair process and put the horse at risk of more serious musculoskeletal injury.This study investigated injury in different breeds of horses involved in various types of activity in riding clubs in Tehran. Of the 400 horses that took part in various events during the race season from March 2008 to September 2009, 26 were injured. The most common injury was to the musculoskeletal system of the fore limbs (64%). The hind limbs suffered 16% of the injuries and the head and neck 4%. From a gender aspect, incident rates were higher in mares (54%) than in stallions (46%). From a breed point of view, Thoroughbred and Arab horses had higher incident rates (100% and 85% respectively) than a hybrid horse (50%). Findings of this study also showed the relationship between gender, breed and age of the horses to the injury site, type and outcome, and type of the events.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 2880

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    21-29
Measures: 
  • Citations: 

    0
  • Views: 

    854
  • Downloads: 

    189
Abstract: 

The aim of this study was to investigate the effect of bacterial inoculation on the fermentation and aerobic stability of corn silage and on the dry matter degradability and milk composition when fed to sheep or cows. Four male sheep were selected to measure dry matter degradability of uninoculated (UCS) and inoculated (ICS) corn silage. Milk composition was measured for 22 Holstein cows, separated into two treatment groups and fed with either UCS or ICS diets.Similar lactic acid concentrations but higher propionic and lower acetic acid concentrations were recorded for ICS diets compared to UCS diets (p<0.05).Aerobic stability was 12 h and 32 h for UCS and ICS, respectively. Values of in sacco dry matter loss were higher for ICS than for UCS (p<0.05). Lactose and solid non-fat content was higher in the milk of cows fed with ICS than UCS (p<0.05). We conclude that the Lalsil bacterial inoculant containing propioni bacterium acidipropionici and Lactobacillus plantarum can be used as an additive due to its positive effect on fermentation, aerobic stability and dry matter degradability of corn silage. The slight positive effect of inoculcation on the nutritional value of silage appears to be limited to milk production improvement in dairy cows.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    31-35
Measures: 
  • Citations: 

    1
  • Views: 

    1253
  • Downloads: 

    273
Abstract: 

Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, conventional culture and multiplex PCR methods were compared for the detection of C. jejuni isolated from poultry carcasses. A total of 100 samples, representing 20 broiler flocks, were collected from poultry carcasses after the evisceration stage in the processing line at a commercial broiler slaughtering facility in Mashhad, Iran. In the conventional culture method, samples were processed by enrichment followed by selective plating, and then suspected colonies were isolated on sheep blood agar and tested for morphology, motility, Gram staining, biochemical properties and hippurate hydrolysis activity. For the identification of the Campylobacter genus and its jejuni serovar by molecular methods, a multiplex PCR assay (m-PCR) with two sets of specific primers was used. In the hippurate hydrolysis test of suspected colonies, 76% of the samples were determined as positive, while in the m-PCR assay 28% of cultures harvested were identified as C. jejuni. Two percent of hippurate hydrolyze negative colonies were found positive in the m-PCR test.It appears that the conventional method, based on the hippurate hydrolysis test for detection of C. jejuni, is a less reliable test. The use of the m-PCR method, based on amplification from conserved genes, allows reliable detection and identification of C. jejuni.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    37-41
Measures: 
  • Citations: 

    0
  • Views: 

    224
  • Downloads: 

    185
Abstract: 

To determine the effect of bilateral uterine artery ligation (BUAL) on mast cell (MC) distribution, 24 mature female rabbits were studied. The rabbits were ovariohysterectomized on day 23, 43 or 63 following BUAL. Histological sections were stained with toluidine blue to determine the MC distribution. In rabbits that had undergone BUAL, MCs were present extensively in the helium of the treated ovaries, in dense groups close to the blood vessels. In the control group, MCs were observed at a medium density around the blood vessels and the number of MCs in the reproductive tract was significantly (P£0.01) lower than in the test groups. This study demonstrates that, after BUAL, the distribution and numbers of MCs differ between different parts of the reproductive system.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    43-47
Measures: 
  • Citations: 

    0
  • Views: 

    1006
  • Downloads: 

    453
Abstract: 

This study was conducted to investigate the hepatoprotective and antioxidant effects of pentoxifylline (PTX) against aflatoxin B1 (AFB1) exposure in perfused rat livers by evaluating damage marker enzymes, antioxidant defense systems (glutathione, GSH) and lipid peroxidation (malondialdehyde, MDA). Sixteen rats were divided randomly into four experimental groups: control, PTX, AFB1 and AFB1+PTX. Rats in the control group were infused with Krebs–Henseleit bicarbonate buffer. Rats in the AFB1-treated group received approximately 1 ppm and the PTX- treated group received 100 mg/kg intraperitoneally 24 h before surgery. Alanine aminotransferase and lactate dehydrogenase levels were increased by AFB1 and decreased by PTX. PTX also ameliorated the increased concentration of MDA caused by AFB1. PTX did not compensate for the decrease in GSH caused by AFB1. These results imply that PTX has an antioxidant effect by inhibiting free radicals, and prior treatment with PTX ameliorates the effects of AFB1-induced lipid peroxidation but does not compensate GSH depots.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    49-52
Measures: 
  • Citations: 

    0
  • Views: 

    248
  • Downloads: 

    232
Abstract: 

Leptospirosis is a zoonosis with numerous reservoir hosts. The disease is caused by infection with various serovars of Leptospira interrogans sensu lato. This study was conducted to evaluate the seroprevalence of leptospiral infection in stray cats in Ahvaz (south-western Iran) from April 2007 to June 2008. Blood samples were collected from 102 stray cats and screened for leptospiral infection using the microscopic agglutination test (MAT). Five of the 102 cats (4.9%) were serologically positive for at least one serovar of L. interrogans. The greatest number of reactors was for L. interrogans serovar balum (five serum samples).Antibodies against more than one serovar (namely, serovars balum and australis) were detected in one sample. All positive titers were detected at 1: 100 dilution. The prevalence of leptospiral infection was 5.3% and 4.4% in male and female cats, respectively. There was no significant difference in positive titer prevalence between different sexes but prevalence was significantly different between age groups (P=0.021), as all cats with positive titers were three years of age or more. This is also the first report of infection with L. interrogans serovars balum and australis from cats in Iran.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    53-58
Measures: 
  • Citations: 

    0
  • Views: 

    820
  • Downloads: 

    95
Abstract: 

Infectious bronchitis (IB) is an economically important disease of chickens. Due to the emergence of new variants of infectious bronchitis virus (IBV), the control of IB has become a serious problem for the poultry industry worldwide. In the present study, the nucleocapsid gene (N) and 3' untranslated region (UTR) of two IBVs isolated from Iranian poultry farms were sequenced and compared with other IBV strains. Based on nucleotide identity, the N gene and 3' UTR sequences of Iranian IBVs showed 90% similarity to the commonly used IBV vaccine strains, H52 and H120. However, based on phylogenetic analyses, Iranian IBVs were found to cluster separately from the IBV vaccine strains used in Iran as well as other IBVs isolated in China, Australia and the United States. It was concluded that IBVs circulating in Iran are genetically distinct from IBV vaccine strains that have been used in Iran for many years. Therefore, it is important to develop a new vaccine based on these newly identified strains for controlling IB in Iranian poultry farms.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    59-62
Measures: 
  • Citations: 

    1
  • Views: 

    323
  • Downloads: 

    150
Abstract: 

The major histocompatibility complex (MHC) plays a central role in the control of disease resistance and immune response. Extensive genetic diversity in MHC genes provides a valuable source for genetic improvement, via selection, in many domestic animals. Exon 2 of the class II MHC, termed Ovar-DRB1 in domestic sheep (Ovis aries), has been suggested as important disease resistance and immune response gene. We characterized Ovar-DRB1 in DNA samples from 138 individuals of a population of the Iranian Sangsari sheep breed using PCR-RFLP.Eight DRB1 alleles were identified among Iranian Sangsari sheep, including one previously unrecognized allele. Eight homozygous genotypes were observed: a, b, c, d, f, g, h and N. Genotype bb was the most common pattern (46 of 138).Heterozygous genotypes (ag, cb, cd, bf, and bN) were also observed. The observed homozygosity and heterozygosity values were 0.6377 and 0.3623, respectively, vs expected values of 0.220 and 0.779. Iranian Sangsari population deviate significantly from the theoretical proportions (FIS= 0.5283; p=0.0005). In conclusion, PCR-RFLP analysis allows rapid identification of Ovar-DRB1 types and discrimination of homozygous and heterozygous genotypes. This study indicates that the exon 2 region of the Ovar-DRB1 gene is highly polymorphic in the Iranian Sangsari sheep breed.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2011
  • Volume: 

    5
  • Issue: 

    1
  • Pages: 

    63-67
Measures: 
  • Citations: 

    0
  • Views: 

    340
  • Downloads: 

    174
Abstract: 

Due to the range of its constituents, colostrum has been considered as a supplement for various diverse purposes. This study was conducted to examine the effect of supplementary bovine colostrum on serum malondialdehyde (MDA), antioxidant activity (AOA) and glucose in a diabetic rodent model. Sixty male Wistar rats were divided into 10 groups of six rats each for 40 days as follows: non-diabetic; diabetic; diabetic with 10%, 20% or 30% colostrum intake; non-diabetic with 10%, 20% or 30% colostrum intake; diabetic treated with insulin; and diabetic treated with glibenclamide. Although serum MDA levels showed a significant decrease in response to insulin (2.56 ± 0.31 mmol/L) and 10%, 20% or 30% colostrum intake (0.46 ± 0.04, 0.29 ± 0.06, 0.37 ± 0.09 mmol/L, respectively), the decrease was greater in the diabetic rats (3.92 ± 0.29 mmol/L) (p< 0.01). Significant changes were seen in the AOA of both insulin (0.78 ± 0.11 mmol/L) and glibenclamide (0.7 ± 0.08 mmol/L) treated rats compared to the diabetic rats (0.69 ± 0.1 mmol/L); however, AOA showed a significant increase in response to 10% (1.78 ± 0.11 mmol/L), 20% (1.57 ± 0.02 mmol/L) and 30% (1.75 ± 0.02 mmol/L) colostrum (p<0.001). All treated groups showed a significant decrease in serum glucose levels compared to the diabetic group (391 ± 39.79 mg/dL) (p<0.01). It seems that colostrum might be a beneficial dietary supplement for reducing serum MDA and glucose levels while increasing serum AOA in type1 diabetes mellitus.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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