The purpose of the present study is to ascertain the relationship of job satisfaction with organizational commitment and mental health between Aligarh Muslim University (A.M.U) and University of Sistan and Baluchestan (U.S.B) employees. The sample size consists of 205 employees (104 employees from A.M.U and 101 employees from U.S.B), randomly selected for this study. Job Satisfaction Questionnaire, Organizational Commitment Scale and General Health Questionnaire (GHQ – 12) were applied to collect the data. Results demonstrated that job satisfaction had significant positive relation with affective, continuance, normative and total scores of organizational commitment. But, job satisfaction was negatively correlated with physical symptoms, anxiety, social dysfunction, depression, and total scores of GHQ – 12. The results of stepwise regression revealed that in A.M.U employees total scores of organizational commitment accounted for 21.9% of the variance and in the second step total scores of mental health accounted for 8% of the variance in job satisfaction, organizational commitment was a significant positive predictor for job satisfaction while mental health was a significant negative one for it. In U.S.B employees total scores of organizational commitment accounted for 29.1% of the variance and in the second step total scores of social dysfunction accounted for 5.7% of the variance in job satisfaction, organizational commitment was a significant positive predictor for job satisfaction, social dysfunction was a significant negative one for it. Results of t-test showed that U.S.B employees had significantly higher scores on depression and mental health in comparison with A.M.U employees. But there was no significant difference between A.M.U and U.S.B employees on job satisfaction, affective, continuance, normative commitment, total scores of organizational commitment, physical symptoms, anxiety, and social dysfunction. Eventually, it can be concluded that the amount of mental health of A.M. U employees were better than U.S.B employees.