The recent long successive droughts in the southern part of Iran, including Fars province, necessitate the need for water resources management. The wide changes in climate variables in the daily, monthly and yearly scales show the relationship of these factors with the atmospheric and oceanic teleconnection patterns such as ENSO, Arctic Oscillation (AO), the Antarctic oscillation, North Atlantic Oscillation (NAO), Madden-Julian Oscillation (MJO) etc. Madden-Julian oscillation, Oceanic Remote Link phenomena and stable patterns are caused by general circulation of the atmosphere. This phenomenon is mainly detected by an area of strong convection with the intensification in rainfall over the Indian and Pacific Ocean. The positive anomalies of rainfall first started in some parts of east Africa and West Indian Ocean and then spread to the east. Many researches in this field have mainly focused on the relationship between temporal and spatial distribution of precipitation with MJO event's status. However, there has been less attention to influence of the MJO on runoff in Iran. While the amount of runoff is one of the main components of the hydrological cycle and plays key role in water resources management, agricultural planning, optimal utilization of dams etc. Here, the main objective is studying the influence of various phases of the MJO on rainfall and rivers runoff in Fars province, Iran. The daily data of hydrometric stations (Doroodzan, Pol-Khan, Cham-Chit, Boshigan, Chiti), and rain gauge station (Boshigan, Chiti, Chamriz, Farashband, Saad-Abad and Jare-Bala) were collected from the Fars Regional Water Office. Then, the stations which had at least twenty years data and the minimum number of missing data were used for analysis. In this study, the focus was on the critical period of Madden-Julian Oscillation in the rainy seasons (October to March) from 1975 to 2015. The daily MJO index from Bureau of Meteorology was used to select critical period of eight phases of MJO, with conditions as the magnitude of the MJO index remain larger than 1 and in the similar phase at least for five days. Also, ANOVA test were used to study the significant effect of eight phases of the MJO on the amount of rainfall and runoff in the rain gauges and hydrometric stations. Then, LSD test was used to compare the differences between averages of the each selected groups. Finally, deviation and percentage deviation from the mean value were used to quantify the impact of each phase on the precipitation and runoff. The results of ANOVA test for rain gauges stations in Sa'd Abad, Jere-Bala, Farashband, Boshigan, Chiti and Chamriz stations showed that the null hypotheses (equality of rainfall means in MJO phases) is rejected. Accordingly, we can conclude that the impact of MJO on the rainfall of selected stations is significant. This test for hydrometric stations of Pol-Khan, Dorood-Zan, Cham-Chit, Chiti, Chamriz and Boshigan showed the significant impact of Madden-Julian oscillation on the rivers runoff in Fars Province, too. In this study, null hypothesis of equality mean of runoff in hydrometric stations was rejected and LSD test confirmed the significant differences between the mean values in eight phases of the MJO. The result obtained from the hydrometric stations of Chiti and Boshigan showed that the phase 8 and 5 of the MJO had the greatest positive and negative impact on the rivers runoff of the, respectively. In Doroodzan and Pol-Khan hydrometric stations, the greatest negative impact on runoff was observed in phases 5 and 7 of the MJO. The analysis of rain gauge data of Boshigan and Chiti showed most precipitation (relative the mean value) in the phase 8 of the MJO, which indicate positive impact of this phase of madden-Julian oscillation on rainfall value. The most negative deviation from the mean value was seen in the Phases 3 and 4 of the MJO in the stations of Boshigan, Sa'd-Abad and Chiti, respectively, which shows the negative impact of the MJO. In Farashband station of Dalaki River, phases 7 and 8 had the greatest positive and negative impact, respectively. Generally, statistical analysis showed that there is a significant difference between the mean of rainfall and runoff value in various phases of the MJO in the selected rain gauge and hydrometric stations. In summary, the analysis of the results in the hydrometric stations showed that the phases 1 and 8 of the MJO have the greatest positive impact and the phase 5 has the greatest negative impact on rainfall. For rain gauge stations, the results showed that the phases 7 and 8 of the madden-Julian oscillation has the most positive impact on rainfall amount, while the phases of 3 and 5 of this oscillation has the most negative impact. So, it is reasonable that at first MJO causes to increase precipitation amount in south of Iran in phases 7 and 8, then in two later phases (8 and 1) the increase in runoff value is due to the lag time in converting rainfall to runoff.