The anatomical and chemical properties of date palm tree fronds (leaves), which are gathered from date palm tree annual pruning, were investigated in this study. The palm used in this research was Stamaran or Sayer cultivar, which is the most predominant cultivar in Khuzestan province. To study the anatomical and chemical properties of the palm frond, the TAPPI standard was used. Leaf Stalk fiber length, diameter, lumen diameter, and cell wall thickness were measured at 1393.66 mm, 18.1 mm, 7.65 mm, and 5.23 mm respectively and leaflet fibers were measured at 1413.71 mm, 15.18 mm, 7.06 mm, and 4.06 mm respectively. Chemical composition was measured for both leaflet and leaf stalk. Chemical composition of the leaf stalk was as follows: cellulose 38.26%, lignin 22.53%, hemicellulose 28.17%, extractives 5.08%, and ash 5.96%. Chemical composition of the leaflet was as follows: cellulose 40.21%, lignin 32.2%, hemicellulose 12.8%, extractives 4.25%, and ash 10.54%. The slenderness ratio, flexibility ratio, and raunkel ratio calculated in Leaf Stalk was 77, 42.26%, and 136.73% respectively and in Leaflet was 93.13, 46.5%, and 115% respectively. It is important to know about these parameters for making composite products.