Due to differentiation ecosystem units and related soil physico - chemical and biological characteristics, 268.7 ha-1 of lowland forests in Khanikan were studied. Vegetation data and information (trees, shrubs, and herbs) were collected from 60 sample plots with systematically random method (20m×20m) which were then analyzed by using TWINSPAN program. The data were classified into five ecological groups. Some of physical, chemical and biological characteristics of soil, such as soil acidity (pH), bulk density, saturation moisture, electrical conductivity (EC), organic carbon, total nitrogen, cation exchange capacity, available phosphorous, soil texture, lime, biomass of earthworms, litter carbon, and litter nitrogen were measured. Principal component analysis (PCA) was used to determine correlation as well as distribution in each ecological group of environmental factors. It was noticed that 1st, 3rd, 4th ecological groups had the highest correlation with the 1st axes whereas 2nd and 5th ecological groups demonstrated the highest correlation with the 2nd axes. The results indicated that the ecological group’s distribution pattern was mainly related to soil characteristics such as pH, bulk density, texture, phosphorous, organic carbon, nitrogen, and CEC.