To comparison of micronutrient application methods effect on grain and straw yield, and protein and elements concentration, a RCBD design with 5 treatments (soil apply, leaf spraying, seed treatment, soil applying+leaf spraying and seed treatment+leaf spraying) in 3 replications was conducted at Tikmedash research station, Miyaneh, Iran. Regarding Verthic Haploxrepts soil analysis results, N, P, and K was applied from urea, Triple super phosphate and potassium sulfate bases respectively. Half of urea was dressed at seed elongation and heading stages. In soil applying method, MgSO4, ZnSO4, MnSO4, CuSO4, EDDHA and H3BO3 was applied as 50, 40, 30, 20, 10, and 20 kg/ha, respectively. In leaf spraying method, micronutrient manures such as ZnSO4, MnSO4, CuSO4, FeSO4 and H3BO3 in 0.002 concentrations+0.05% urea was applied in stem elongation, heading and a week after anthesis stages. In seed treatment method a stock solution in 30% concentration of all above mentioned nutrients was prepared and sprayed on 10 kg wheat seeds, and then the seeds were sown. In soil application+leaf spraying method the 1 and 2 methods in leaf spraying and the 2 and 3 methods in seed treatment was used. During growth stages 5 irrigation was applied by sprinklers. Statistical analysis showed that the soil application+leaf spraying method increased grain and straw, protein contents and seed elements concentrations significantly, and so could be recommended. in wheat cultivation