In this work, we collected and determined medicinal, aromatic, pastural and rare plants species from three protected regions of the Isfahan province including Kolah-Ghazi's National Park, Mouteh and Ghamishlou Wildlife Sanctuaries, located respectively in 26 Km SE, 95-155 Km NW and 25 Km NW of Isfahan city. Analyses of the flora of these regions show that, there are 459,511 and 493 vascular plants species respectively in the Kolah-Ghazi, Mouteh and Ghamishlou areas. Main natural and floristic characteristics of these regions are reported briefly. The results of these study show that, in total, about 90 medicinal, aromatic and industrial plant species, about 44 grasses, 22 legumes, 53 forbs, and 23 tree and shrub species exist in these protected areas. Furthermore, threatened species of these regions have been analysed, according to the IUCN criteria. The most important criteria for determination of the rare species categories have been evaluated. On the basis of this study, four categories of rare species so called endangered, vulnerable, lower risk and data deficient are determined and list of these species have been presented. This study show that, there are in total, 54 threatened plant species in this three protected areas. Finally, we discussed similarities floristic composition, and species richness of these areas.