Well-structured multi-dimensional and consists of emotional, cognitive, experience positive and negative emotions such as frustration and discomfort, and judging from the quality of individual lives. Psychological well-being criterion not only reflects child affections but also demonstrates aspects that person's life. The purpose of the present study was to develop and study characteristics of psychometric properties of suitable questionnaire to measure well-being of preschool children among their mothers. The research method of study was correlational and validation through developing its reliability. The sample consisted of 580 children that were selected by cluster multistage random sampling method. Preschool children’s well-being questionnaire was evaluated by construct validity (explanatory factor analysis and internal consistency), convergent validity and reliability (internal consistency) and Pearson correlation index in study group. As exploratory factor analysis of the five dimensions of well-being were extracted which including psychological, social, cognitive, educational, and family. As well as significant correlation between children's well-being of questionnaire and Q-set Questionnaire scores confirmed convergent validity.The reliability of each of the subscales of psychological, social, cognitive, educational, family and general well-being questionnaire preschoolers by Cronbach's alpha, respectively were 0.73, 0.83, 0.77, 0.70, 0.73, and 0.89. According to results of this research, the preschool children’s well-being questionnaire had good validity and reliability which can be used for evaluating preschool children’s well-being.