In order to study the impact of water deficiency stress on the physiological characteristics and grain yield of oil sunflower during the years of 2004-2005 in Agricultural Research staition of Khoy, an experiment was done in split plot on the basis of randomized complete block design in four replications. The irrigation levels, as the main factor in 6 levels (including 70-70 -70, 90 -90 -90, 110-110-110, 90-70-90, 110-70-110, and 110 -90 -90 mm of evaporation from class A evaporation tray were put into action, respectively, from shrub settlement stage to the appearance of head, the appearance of head till the start of turning into yellow and the start of turning to yellow till to physiological ripening and the cultivars including: Hisun33 Records and Armaverski were chosen as sub factor. The results of the research showed that with increasing in the alterations of irrigations, there was reduction in length ripening period, leaf area index and relative water content of leaves, grain and oil yield, and there was increased in proline amount of leaves. The irrigation levels of 70- 70-70 and 90-70-90 mm evaporation with LAI= 4.7 at pollination stage produced the most grain and oil yield respectively: 5125 and 2382 kg per hectare. Record and Hisun33 cultivars with desired ripening stage, LAI and RWC, had the highest grain and oil yield in comparison with Armaverski cultivar. With regards to significant interactions between experimental factors concerning with LAI, grain and oil yield with using deficiency stress and irrigation after 110 mm evaporation decreased leaves development and the same affair led to the reduction of grain and oil yield, because the lowest amounts of three mentioned traits (LAI, grain and oil yield) was observed in irrigation treatment after 110 mm.