In order to achieve uniform emergence and the higher yields of Citrulus colocynthis. As a medicinal plant, breaking seed dormancy is an essential factor. In this context, regarding the medicinal value of this plant, this experiment was conducted with 20 treatments based on completely randomized design with four replications on the one Citrulus colocynthis ecotype at Islamic Azad University, Khorasgan Branch in 2009. Treatments which used in this experiment were included: control treatment- scratching- 24, 48 and 72 hours washes and two, four, six, eight and 10 weeks putting seeds in the cold conditions. Potassium nitrate in six levels of 0.1, 0.2, 0.3, 0.4, 0.6, 0.8 and 1 percent, and a normal sulfuric acid, at different times for 2 minutes, 5 minutes, 8 minutes and 11 minutes were used as well. The traits include: percent of germination, germination rate, the mean root length, mean length stem let, the fresh and dry weight of root, the fresh and dry weight of stem let, and the root shoot allometric coefficient were calculated and analysis of variance was performed for each one of traits. Results showed that all treatments used in this experiment had a positive effect on breaking dormancy, except using of 11 minutes sulfuric acid. The highest germination rate and percent of germination was gained by using of 5 minutes of Sulfuric acid there were no significant differences between using of 5 minutes of Sulfuric acid, Potassium nitrate0.4% and scratching treatments related to percent of germination trait. It seems that hard seed coat in this plant is most important factor to prevent seed germination and seed dormancy in this plant to look to be a kind of induction dormant.