Knapweed (Acroptilon repens L.) is a highly competitive weed in the family of sunflower (Asteraceae). Using systemic chemicals is one of the most important methods to control perennial weeds such as Russian knapweed. This study (during 2005-2006) was performed in experimental greenhouse of Agriculture, Medicine and Industrial Research School of Nuclear Technology of Karaj. Experiments were conducted to examine the influence of Russian knapweed growth stage on 2, 4-D and Glyphosate absorption and translocation. Russian knapweed plants were inoculated with 14 C-2, 4-D and 14 C-glyphosate with activity of 0.080 and 0.085 micro curi (in each 10 ml of solution) in rosette, bud and bloom growth stages. This experiment was arranged as factorial with four replications in a randomized complete block design with four replications. Factors were herbicide (2,4-D and glyphosate) and Russian knapweed growth stage (rosette, bud and bloom stages). Extraction and counting of labeled herbicide (After 168 hours) showed that, there was no significant difference between translocated amount of 2, 4-D and glyphosate. But, Root accumulation of 14 C-2,4-D by Russian knapweed was greater in rosette stage, and root accumulation of 14 C-Glyphosate was greater in bud and bloom stages. Results of this study showed that root accumulation of 2, 4-D and glyphosate are directly dependent to ability of Russian knapweed control. It is expected that the best application time to chemical control of Russian knapweed by 2, 4-D and glyphosate is at rosette and bloom stages, respectively.