Several factors cause the degradation of cellulose and paper subsequently; among them, acidic hydrolysis is the most important one. Many historical books and documents are gradually weakened and destroyed due to acidic hydrolysis and reduction of the degree of polymerization of cellulose. So, deacidification and strengthening treatments are necessary for valuable old papers to stop these processes. Since the paper is a good substrate for growth of some destroying fungi, applying a suitable restoration treatment to stop or delay the growth of these destroying microorganisms-in addition of increasing the pH and preparing alkaline conditions for paperhas been considered. In this study, in order to deacidification and strengthening of paper, with different combinations of nano calcium hydroxide, nano hydroxyapatite, nano cellulose and nano chitosan in an alcoholic solvent )ethanol), a nano coat was applied on the surface of the sample paper. To study the anti-fungal properties, biological aging was done on treated paper with a fungus (Aspergillus niger). For this purpose, after preparing culture medium, treated papers were inoculated in separate petri dishes and after incubation period, the amount of fungal growth was studied. Also, tensile strength test was done on treated samples. Results of antifungal and tensile strength of treated papers showed that deacidifation treatments by preparing an alkaline condition increased the pH of paper and the paper treated with a complex of nano calcium hydroxide and nano chitosan, and also treated samples with nano hydroxyapatite had the lowest fungal contamination. Treated papers with nano calcium hydroxide and nano chitosan also showed better tensile strength properties. Although applying nano cellulose in treatments had the less anti-fungal resistance, but improved the tensile strength of treated papers.