In this study, the effects of sesame oil on spatial learning have been investigated. Male rats were trained for a spatial learning task using T-Maze. The rats divided into four groups and 8 rats were considered for each group. The first group, control group wad fed with an ordinary diet and other groups were fed with a diet containing sesame oil (10%) for two, three and four weeks. All rats were trained for 9 days based on standard method. Results showed an increase of spatial learning in rats fed (10%) sesame oil in their diet for four week (p<0.05), but significant was not for two and three weeks. The sesame oil consists of unsaturated fatty acids and phosphatidil cholin (lecithin) that can decrease cholesterol. It seems that it has a molecular role in spatial learning in rats. Probably, the change in activity cholinergic system is as a result of existence of lecithin component in sesame oil.