Inroduction & Objective: Opioidergic system has crucial role on central regulation of food intake in mammals and birds. This study was designed to investigate role of opioidergic system on opioid-induced feeding behavior in 3-h food-deprived (FD3) neonatal layer-type chicks. Material and Methods: 144 chickens allocated into3 experiments, each with four treatment groups. In Experiment 1, birds were ICV injected with D-Ala2– NMe-Phe4-Glyol5-enkephalin (DAMGO, μ-opioid receptor agonist) (125, 250 and 500pmol). In Experiment 2, chickens were ICV treated with D-Pen2, 5Enkephalin, DPen2, D-Pen5Enkephalin (DPDPE, δ-opioid receptor agonist) (20, 40 and 80pmol). In Experiment 3, the effect of ICV injection of U-50488H-, κ opioid receptor agonist (10, 20 and 30nmol) was investigated in chicks. Cumulative food intake recorded until 3-h post injection. Results: According to the results, ICV injection of μ-opioid receptor agonist significantly diminished food intake (P≤ 0. 05). Also, injection of δ and κ-opioid receptors agonist significantly increased food intake (P≤ 0. 05). ICV injection of DAMGO significantly decreased food intake whereas DPDPE and U-50488H increased food intake (P≤ 0. 05). Conclusion: These results suggest that central opioidergic system has important role on feeding behavior.