Avicenna Journal of Phytomedicine
Year:2020 | Volume:10 | Issue:4|Papers Count:10

Objective: This study aims to evaluate the effects of onion (Allium cepa L. ) against hyperglycaemia and dyslipidemia and determine possible changes in these effects due to different heat treatments applied to onion. Materials and Methods: 32 male Wistar-albino rats were divided into 4 groups as follows: the groups C and DC were fed with standard rat diet; the DLO group was fed with rat diet including 5% onion powder dried at-76° C in a lyophilizator, and the DFO group was fed with rat diet including 5% onion powder dried at 80° C in a furnace. Diabetes was induced in DC, DLO and DFO groups by injection of streptozotocin (45 mg/kg). Results: A decreasing tendency was observed in fasting blood glucose (FBG) values of DLO group during the experiment period and it was found that the 6th and 8th weeks values were significantly lower than the 1st and 2nd weeks values (p<0. 05). On the other hand, no statistical difference was observed in the FBG values measured at different weeks in the DFO group. Significant differences were also observed among the groups in terms of plasma lipid values. DLO group was determined to have lower levels of triglyceride (p<0. 001), LDL cholesterol and total cholesterol and higher levels of HDL cholesterol (p<0. 05 for all cases) compared to the DC group whereas no significant difference in these values was found between the DFO and DC groups. Conclusion: Lyophilized onion powder may be protective against hyperglycaemia and dyslipidemia arising from diabetes. However, the heat treatments applied to onion affect this protective role negatively.

_Methamphetamine (METH) increases dopamine, norepinephrine and serotonin concentrations in the synaptic cleft, and induces hyperactivity. The current management of acute METH poisoning relies on supportive care and no specific antidote is available for treatment. The main objective of this review was to present the evidence for effectiveness of the herbal medicine in alleviating the adverse effects of METH abuse. Materials and Methods_ Literature search was performed using the following electronic databases_ MEDLINE, Scopus, PubMed and EMBASE. Results_ Plant-derived natural products ginseng and sauchinone reduced METH-induced hyperactivity, conditioned place preference and neurological disorder. Garcinia kola decreased METH-induced hepatotoxicity, raised METH lethal dose, and restored the METH-impaired cognitive function. Repeated administration of baicalein resulted in attenuation of acute binge METH-induced amnesia via dopamine receptors. Activation of extracellular-regulated kinase in the hypothalamus by levo-tetrahydropalmatine facilitated the extinction of METH-induced conditioned place preference and reduced the hyperactivity. Other herbal medicine from various parts of the world were also discussed including hispidulin, silymarin, limonene, resveratrol, chlorogenic acid and barakol. Conclusion_ Based on the current study, some natural products such as ginseng and levo-tetrahydropalmatine are promising candidates to treat METH abuse and poisoning. However, clinical trials are needed to confirm these finding_

Objective: Numerous studies demonstrated the effect of grape on memory improvement. According to Iranian traditional medicine, “ mavizˮ as a specific type of dried grapes can effectively improve memory. However, there is no reported clinical trial on the effect of “ mavizˮ on memory improvement in humans. Hence, this study was conducted to investigate “ mavizˮ effect on memory in university students. Materials and Methods: This randomized open-label clinical trial was conducted on a total number of 53 students of Shahed University, Tehran, Iran, from November 2017 to February 2018. The participants were randomly allocated into an intervention group (receiving “ mavizˮ , 25 g in the morning for 4 weeks) or a control group (who did not take “ mavizˮ ). The Digit Span Task and the NBack Task were used for the measurement of working memory at the pre-and post-intervention stages. Results: According to the results, no significant differences were found between the two groups regarding age, gender, marital status, and initial working memory test scores. “ mavizˮ consumption produced a highly significant improvement in total working memory score in the Digit Span Task (5. 18 vs. 2. 35, p<0. 001) and Acoustic Memory Span (1. 29 vs. 0. 62, p=0. 021). Moreover, “ mavizˮ consumption significantly increased the percentage of true responses in the N-Back Task and reduced the mean reaction time in the first level of the task. Conclusion: “ mavizˮ consumption was improved working memory in young healthy adults.

Objective: Acute and chronic ethanol consumption cause oxidative stress and ginger improves suchconditions. In this study, the protective effects of ginger were studied on indices of oxidative stress, total homocysteinelevel and the level of the some of the oxidative stress-associated trace elements against toxicity induced by ethanol in rat eye. Materials and Methods: Twenty-four adult male Sprague-Dawley rats were randomly allocated into four groups and treated daily for 28 days as follows: group I: control; group II: ginger (1g/kg/day ginger extract by oral gavage); group III: ethanol (4g/kg/day ethanol by oral gavage) and group IV: ginger+ethanol. At the end of the experimental period, eye tissue sera were used for determination of different parameters. Furthermore, in vitro antioxidant potential and total phenol content of ginger extract were determined. Results: In ethanol group, significant changes in oxidative stress markers and levels of homocysteine and some trace elements, compared to other groups, were observed (p<0. 05 for all cases). However, these parameters significantly ameliorated with pretreatment with ginger in ginger+ethanol group (p<0. 05 for all cases), and had no significant differencesinthese parameters betweenginger and control group were found. Conclusion: It can be concluded that ginger extract has protective effects against toxicity induced by ethanol in the eye of male rat.

Objective: The long-term sequelae of methotrexate (MTX) remain the major cause of concern for both patients and therapists. Therefore, new approaches to decrease MTX side effects are needed. The study was carried out to evaluate the effects of Iris songarica Schrenk (IS) rhizome extract against MTX-induced hepatic and renal injuries in rats. Materials and Methods: Forty male Wistar rats were randomly divided into five groups (n=8) including control, MTX, IS50, IS150 and IS300. Control and MTX groups were only treated orally with saline; whereas, IS50, IS150 and IS300 groups were treated with IS extract at three different doses (50, 150, and 300 mg/kg, respectively). Besides, the MTX and experimental groups were received a single dose of MTX (20 mg/kg) intraperitoneally on day 4. On the ninth day, animals were sacrificed, blood transaminases, urea and creatinine were assessed and the concentration of malondialdehyde (MDA) and the activity of super-oxide dismutase (SOD) were determined in both liver and kidney tissues. Moreover, hepatic and renal damages were evaluated histopathologically. Results: MTX by increasing oxidative stress (MDA) and decreasing antioxidant capacity (SOD) induced hepatic and renal damages as confirmed by biochemical and histological parameters analyses. However, treatment with IS caused significant improvements in hepatic and renal histological architectures and SOD activity (p<0. 01) along with reducing liver enzymes, urea, creatinine and MDA (p<0. 01). Conclusion: The results of the present study showed that IS extract through antioxidant and probably anti-inflammatory activities, could effectively limit MTX-induced hepatic and renal injuries in rats.

Objective: Inhibition of lipid metabolism in breast cancer has been suggested as an effective approach for cancer therapy. Saffron-derived crocetin (Crt) and crocin (Cro) with the known anticancer activity, have shown hypolipidemic effect in diabetes and atherosclerosis. Here, we investigated the effect of Crt/Cro on lipid content in breast cancer. Materials and Methods: A multi-model approach involving in vivo, in vitro and in silico studies was applied. The 4T1-induced breast cancer in mice was used to investigate the effect of Crt/Cro on cholesterol (Chl) and triglyceride (TG) levels in serum and tumor tissues. The Chl/TG levels were also assessed in the cytosol of MDA-MB-231 and MCF-7 breast cancer cell lines 6, 12 and 24 hr after Crt/Cro treatment. The interaction between Crt/Cro and hydroxymethylglutaryl coenzyme A reductase (HMGCR) was also computed by docking analysis. Results: Crt reduced both serum (p=0. 003) and tumor (p=0. 011) Chl and TG (p=0. 001) levels in mice. Cro reduced TG levels in tumor (p=0. 014) and serum (p=0. 002) and Chl level in tumor (p=0. 013) tissues. Crt reduced both Chl and TG in MDA-MB-231 (p=0. 014 and p=0. 002, respectively) and MCF-7 (p=0. 014 and p=0. 002, respectively), after 24 h. Cro reduced both Chl and TG in MDA-MB-231 (p=0. 014 and p=0. 002, respectively) and MCF-7 (p=0. 014 and p=0. 002, respectively), after 24 h. Crt binds to the active site of HMGCR with higher affinity (Δ G0=-6. 6 kcal/mol) than simvastatin (Δ G0 =-6. 0 kcal/mol). Conclusion: Crt and Cro effectively decreased Chl/TG content in the sera of tumor bearing mice, in breast tumors and breast cancer cell lines. Crt showed a higher hypolipidemic potential than Cro. In silico analysis indicated Crt binding in the HMGCR active site.

Objective: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. Materials and Methods: In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1. 5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay. Results: Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26. 53%, 34. 92%, and 31. 38%, 1, 2, and 5 hr after GSE ingestion (p<0. 001), respectively; this variable in group 2 was 17. 38, 38. 33, and 31. 38 (p<0. 001), in group 3, was 35. 65%, 46%, and 37. 15% (p<0. 001), respectively and in group 4, was 41. 35%, 51. 73%, and 50. 55% (p<0. 0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51. 73%) was observed 2 hr after ingestion of 1000 mg GSE. Conclusion: Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity.

Objective: In a previous work, we showed that asafoetida essential oil (AEO), from oleo-gum resin of Ferula asafoetida L. from the Apiaceae family, has a vasodilatory effect. This effect was both endothelium-dependent and endothelium-independent. The present study was designed to determine whether potassium channels and intracellular calcium release contribute to AEO-induced vasodilation. Materials and Methods: Rats' thoracic aorta were isolated and denuded. Following induction of contraction by potassium chloride (60 mM), concentration-response curve was plotted by the cumulative addition of AEO (0. 625-80 μ l/l to the medium of rings. The vasodilatory effect of AEO was assessed before and after addition of phenylephrine and potassium channel blockers (including barium chloride (BC), 4-aminopyridine (4A) and glibenclamide (Gl)). Results: AEO relaxed the precontracted rings in a concentration-dependent manner (IC50=23 μ l/l). All potassium channel blockers significantly attenuated the vasodilatory activity of AEO when they were added to rings medium before addition of KCl (p<0. 01, 4A and Gl groups and p< 0. 001, BC group vs. control group) but not after that. In contrast to K channel blockers, adding AEO before or after phenylephrine, the tension was reduced significantly (p<0. 05 vs. the control group). Conclusion: The findings of this study indicated that the vasodilatory effect of AEO on denuded-endothelium aortic ring was mediated through activation of potassium channels and reduced intracellular calcium release.

Objective: The present study was aimed to study anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Materials and Methods: Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli) induced secretion in rat ileal loop model. The effect of PHF (12. 5-100 μ g/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells. Results: PHF demonstrated significant (p≤ 0. 05) anti-diarrhoeal activity by increasing the time for first faecal drop and inhibited diarrhoeal episodes by 43, 58 and 60% at 75, 150 and 300 mg/kg body weight, respectively in a dose-dependent manner. Also, the intestinal transit was inhibited upto 33% and the weight of secretory contents induced by castor oil was significantly reduced by PHF, approximately 29% in enteropooling assay. On the other hand, the intestinal loop instilled with PHF and enterotoxin from E. coli demonstrated 61% inhibition of fluid accumulation as compared to loop instilled with enterotoxin only. In vitro studies indicated that PHF inhibits cAMP release in HT-29 cells corroborating the anti-secretory effects observed in aforesaid studies. Conclusion: The results suggest that the PHF possesses anti-diarrhoeal activity, evident through reduced faecal output, decreased intestinal transit and anti-secretory activities.

Objective: Bixa orellana is a plant from the Bixaceae family, for which, limited information is available on hepatoprotective properties. This study aimed at evaluating the protective effects of this plant on sub-acute acetaminophen (APAP)-induced liver injury in mice. Materials and Methods: Various aqueous extracts were prepared from roots, leaves, and barks. Albino mice were divided into six groups: a control group, an APAP group; a silymarin group (positive control) and three test groups. Mice were treated orally with APAP (250 mg/kg) followed 3 hr later by plant extracts, silymarin (50 mg/kg) or distilled water (10 ml/kg) administration once daily, for seven days. After treatment, animals were sacrificed, the liver was collected and different biochemical parameters were measured. Histological analyses were performed using hematoxylin/eosin staining and the qualitative phytochemical content of plant extracts was evaluated using conventional methods. Results: Administration of B. orellana barks decoction (250 mg/kg) significantly reduced alanine aminotransferase levels (p<0. 001), unlike leaves and roots extracts. Moreover, the bark infusion had the highest activity compared to macerate and decoction. It significantly reduced malondialdehyde levels (p<0. 001) and increased the levels of glutathione, superoxide dismutase and catalase, at doses of 250 and 500 mg/kg compared to the APAP group. A significant (p<0. 001) decrease of tumor necrosis factor-α levels and leukocyte infiltration was found following treatment with bark infusion. The infusion content evaluation revealed the presence of polyphenols, saponins, tannins, sterols, anthraquinones, and coumarins and the absence of alkaloids. Conclusion: These results show that infusion from B. orellana barks is hepatoprotective against APAP-induced toxicity via antioxidant and anti-inflammatory mechanisms.