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Information Journal Paper

Title

Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms

Pages

  417-427

Abstract

 Objective: The present study was aimed to study Anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Materials and Methods: Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli) induced secretion in rat ileal loop model. The effect of PHF (12. 5-100 μ g/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells. Results: PHF demonstrated significant (p≤ 0. 05) Anti-diarrhoeal activity by increasing the time for first faecal drop and inhibited diarrhoeal episodes by 43, 58 and 60% at 75, 150 and 300 mg/kg body weight, respectively in a dose-dependent manner. Also, the intestinal transit was inhibited upto 33% and the weight of secretory contents induced by castor oil was significantly reduced by PHF, approximately 29% in enteropooling assay. On the other hand, the intestinal loop instilled with PHF and enterotoxin from E. coli demonstrated 61% inhibition of fluid accumulation as compared to loop instilled with enterotoxin only. In vitro studies indicated that PHF inhibits cAMP release in HT-29 cells corroborating the Anti-secretory effects observed in aforesaid studies. Conclusion: The results suggest that the PHF possesses Anti-diarrhoeal activity, evident through reduced faecal output, decreased intestinal transit and Anti-secretory activities.

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    APA: Copy

    Murugan, Sasikumar, Purusothaman, Divya, Jothie Richard, Edwin, Suresh Chalichem, Nehru Sai, Bethapudi, Bharathi, Chandrasekaran, Prasanna Raja, Chinampudur Velusami, Chandrasekaran, Souza, Prashanth D., & Mundkinajeddu, Deepak. (2020). Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 10(4), 417-427. SID. https://sid.ir/paper/765875/en

    Vancouver: Copy

    Murugan Sasikumar, Purusothaman Divya, Jothie Richard Edwin, Suresh Chalichem Nehru Sai, Bethapudi Bharathi, Chandrasekaran Prasanna Raja, Chinampudur Velusami Chandrasekaran, Souza Prashanth D., Mundkinajeddu Deepak. Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2020;10(4):417-427. Available from: https://sid.ir/paper/765875/en

    IEEE: Copy

    Sasikumar Murugan, Divya Purusothaman, Edwin Jothie Richard, Nehru Sai Suresh Chalichem, Bharathi Bethapudi, Prasanna Raja Chandrasekaran, Chandrasekaran Chinampudur Velusami, Prashanth D. Souza, and Deepak Mundkinajeddu, “Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 10, no. 4, pp. 417–427, 2020, [Online]. Available: https://sid.ir/paper/765875/en

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