In recent decades, in different countries, industrial development and firms expansion in various sizes are caused that industry sector is formed with an emphasis on small and medium firms or large firms. Examining the existence of the relationship between firms’ size and economic growth can affect the related policies that support firms. In the present paper, the effect of small and medium firms on economic growth in Iran, compare to the effect of large firms, is studied during 1987 to 2014 (1366 to 1393 corresponding to Iranian calendar). For this purpose, according to Romer (1990) and Lee (2010) growth model, economic growth is considered as a function of the number of small and medium, and large firms, labor, physical capital accumulation and research & development expenditure. The results which are obtained by using co-integration and Auto regressive vector methods, indicate that the number of small and medium firms and also the number of large firms has a significant positive effect on economic growth. In addition, the number of small and medium firms has a greater influence on economic growth than has the number of large firms. T he reason is due to the short - term return of small and medium firms and also to the existence of many obstacles in relation to the large firms’ growth in Iran' s economy.