Gene, Cell and Tissue
Year:2020 | Volume:7 | Issue:2|Papers Count:7

Dear Editor, Nowadays, neural stem cells (NSCs) are known as an interesting study topic. These ectodermal progenitor cells are generated in the developing central nervous system (CNS) during the fetal period (1). The potential of NSCs to differentiate into neurons (neurogenesis), and glial cells (gliogenesis) will continue for the entire life in the specific areas of brain cortex (2). This potential can be damaged by maternal and environmental stress. Noise stress as an environmental stressor can cause adverse effects in the CNS (3).

Tumor dormancy is defined as a “ steady state” where circulating tumor cells (CTCs) are present in patients but do not form a large vascularized tumor. This is due to the absence of stimulatory signals or prolonged activation of suppressive signaling pathways (1). These metastatic cells have been partially accounted for tumor recurrence even years after initial therapies (2). Hence, as hallmarks of tumor progression, CTCs supply beneficial information about single tumors and involved mechanisms by which these cells shed from the original tumor site (3). Although different technologies have been proposed for CTC isolation, nanomaterials have a good potential in overcoming this hurdle. The cell capturing abilityandunique structure of biomaterials may provide insights for discovering nextgeneration treatments against metastatic tumors....

Background: Escherichia coli is considered as one of the causes of opportunistic infections. Nowadays, due to the increase in drug resistance, the treatment of these infections has become very difficult and they are recognized as the main causes of death in hospitalized patients. Objectives: The aim of this study was to determine the prevalence of blaTEM, blaSHV, and blaCTX-M genes in E. coli strains isolated from the urinary tract infection in patients in Intensive Care Units of three different hospitals in Qom, Iran. Methods: This study was conducted in three months from October to December 2014. A total of 200 E. coli samples were taken from the patients with urinary tract infections in Intensive Care units of Qom hospital. The disc diffusion method was used to determine the susceptibility pattern of antibiotic and phenotypic confirmatory tests for screening of the expanded spectrum beta-lactamase (ESBL) isolates. The presence of blaTEM, blaSHV, and blaCTX-M genes was evaluated by the polymerase chain reaction (PCR) assay. Results: Of 200 samples, ampicillin (96%) and nitrofurantoin (19. 5%) showed the highest and lowest drug resistance, respectively. A total of 156 isolates (78%) were identified as ESBLs using the phenotypic method. Moreover, 76 (38%), 90 (45%), and 123 (61. 5%) isolates consisted of blaCTX-M, blaSHV, and blaTEM, respectively. Conclusions: Overall, the findings of this study showed that blaTEM was the most common gene with a frequency of 61. 5% in ESBL E. coli.

Background: Excessive apoptosis in the myocardium contributes to the pathogenesis of heart disease. Evidence indicates that high-intensity interval training (HIIT) can alter myocardial apoptosis-related signaling. Objectives: The purpose of the study was to investigate the effect of 12 weeks of HIIT on some indices of apoptosis, including Bcl-2, Bax, and caspase-3 in the myocardial tissue of male rats. Methods: Thirty male Wistar rats (age range: 8-10 weeks) were randomized into three groups, including sham (n = 10), control (n = 10), and HIIT (n = 10). The experimental group underwent HIIT consisting of 2-8 repetitions of 4-min high-intensity intervals (85%-90% peak speed) interspersed with low-intensity intervals (45%-50% peak speed), performed five times/week over 12 weeks. Forty-eight hours after the last training session, animals’ hearts were removed. The gene expressions of Bcl-2, Bax, and caspase-3 were analyzed by the RT-PCR method. The independent t-test was used for statistical analysis (P < 0. 05). Results: The gene expressions of Bcl-2 and Bcl-2/Bax ratio were higher in the training group than in the control group (P = 0. 001 and P = 0. 002, respectively). Regarding Bax and caspase-3 gene expression, although the mean levels were lower in the exercise group than in the control group, the differences were statistically insignificant (P = 0. 57 and P = 0. 27, respectively). Conclusions: Overall, it seems that HIIT significantly increases the expression of anti-apoptotic indicator Bcl-2 and the ratio of Bcl-2/Bax in the myocardium of male rats. However, further studies are needed to show the effects of HIIT on myocardial apoptosis.

Dear Editor, Alzheimer’ s Disease (AD) is the most common progressive neurodegenerative disease that affects memory and cognition (1). Nowadays, using an experimental model of neurodegenerative disease is crucial for understanding underlying pathogenesis, disease prognosis, mechanism, and the therapeutic effect of various drugs and obtaining further information (2). I read with great interest the article entitled “ The effect of running on positive and negative slopes on serotonin levels in the hippocampus tissue of rats with Alzheimer’ s disease” by Hassanlouei et al. I would like to thank the authors for sharing their valuable experience on a rat model of Alzheimer’ s disease...

Background: Recent studieshaveshownthat obesityandmetabolic disorders are majorcontributors to heart diseases, andexercise training and proper diets can prevent these diseases. Objectives: The present study aimed to investigate the effect of continuous and interval training with adenosine consumption on adenosine A1 and A2A receptors in the heart tissue of obese rats. Methods: We randomly divided 56 obese rats with a high-fat diet (40% fat, 13% protein, and 47% carbohydrate) into seven groups, including (1) control, (2) continuous training (CT), (3) high-intensity interval training (HIIT), (4) CT + adenosine consumption, (5) HIIT + adenosine consumption (6), adenosine consumption, and (7) sham. Groups 2 and 4 performed continuous training for 12 weeks, 15-31 min at 20-25 m/min, and groups 3 and 5 performed HIIT at 7-10 one-minute intervals at 31-55 m/min and six intervals and active rest at 15-25 m/min. The adenosine consumption groups received adenosine at 0. 2 mg/kg daily in the peritoneum. The independent samples t-test, two-way Analysis of Variance (ANOVA), and Bonferroni’ s post hoc test were used to analyze the data (P  0. 05). Results: Adenosine consumption decreased the levels of A1 and A2A gene expression (P 0. 05). Continuous and interval training decreased A1 gene expression. The combination of training and adenosine consumption significantly reduced A1 gene expression (P  0. 05). Exercise training and the combination of training and adenosine consumption showed no significant effect on A2A gene expression (P > 0. 05). Conclusions: It seems that adenosine consumption decreases the expression of A1 and A2A in the heart tissue of obese rats. Also, training and training with adenosine consumption significantly decrease A1 expression in the heart tissue of obese rats.

Background: Physical activity and dynamic lifestyle play an important role in the prevention and treatment of some common neurological diseases. Onevaluable approach to increasing healthandreducing braindamageis to performanexerciseandphysical activity. Objectives: The aim of the present study was to investigate the effect of exercise pre-training on the intervening factors and complications of cerebral ischemia. Methods: In this study, forty Wistar rats were randomly divided into four groups, including (1) endurance training, (2) endurancecognitive training, (3) ischemia, and (4) control. The training was performed for 3 weeks. After the last training session, the rats had to undergo cerebral ischemia. The ischemia was induced by occlusion of both common carotid arteries for 30 min. Real-time PCR method was used to measure the expression level of TrkB, Bax, and Bcl-2 genes. Results: The levels of TrkB gene expression were significantly increased in the endurance training and endurance-cognitive training groups compared to the ischemia and control groups (P < 0. 05). There was a significant increase in the levels of Bcl-2 gene expression in the endurance training group compared to the ischemia group as well as the endurance-cognitive group compared to the ischemia and control groups (P < 0. 05). Also, the levels of Bax gene expression in the endurance training and endurance-cognitive groups were significantly lower than the control group (P < 0. 05). Conclusions: This study showed that exercise as a preconditioning stimulus has neuroprotective effects against cerebral ischemia.