The synthetic polymers are widely used as adhesives, consolidants and protectivecoatings in the conservation of historic relics. Although, compared to the natural polymersand adhesives, these materials present a better resistance against the microorganisms, mostsynthetic polymers remain appropriate sources of Carbon and energy for the growth ofmicroorganism; in addition, the relics consolidated with these materials will still remainvulnerable to the microorganisms and fungi, leading to a sort of degradation whoseprogress will, nonetheless, be accelerated with the environmental factors and ultravioletlight. Therefore, preventing the microorganism from attacking the relics, as well asincreasing their impact resistance against the environmental conditions are necessary.In this study, in order to maintain the antifungal properties in PolyVinyl Butyral (PVB),TiO2 nanoparticles were employed; anatase-type TiO2 nanoparticles were used in PVB toprepare the nanocomposites. Also, the Samples were made of Poplar wood (Populus spp.)in accord with the Bravery guidelines, treated by the Titanium Dioxide nanocompositeunder vacuum conditions. Afterwards the samples were separately affected in the darkand under the daylight conditions by the wood white-rot fungi (Trametes versicolor) as adeterioration active, for the duration of seven weeks. The treated and untreated sampleswere placed under the accelerated aging condition (temperature, humidity and UV light).The results indicate that the wood samples treated with 1% of Nano-TiO2 (0.4g) in 5%PVB, placed in the dark, did not have any antifungal effect, while the amount of 2%(0.8g) could protect the wood against the white-rot fungus degradation in the darknessconditions. The inoculated samples with the amounts of 1%and 2% of Nano-Ti2O, inthe light conditions, could also create antifungal properties in the treated samples withnanocomposite, and protected the wood against the white-rot fungus. The acceleratedaging of the treated samples with consolidant without nanoparticles of Ti2O and the treatedones with nanocomposite under the heat, humidity and UV light conditions demonstratesthat the treated samples were protected against the degradation, ultraviolet light and theenvironmental conditions.