Fenugreek (Trigonella Foenum Graceum) is one of the medicinal plants that has been used as a folk medicine in Iran and many countries. In this study, effect of macronutrient element shortage (Nitrogen, Phosphorous, Potassium, Magnesium and Calcium) on dry and fresh weight, root and shoot length, leaf relative water, chlorophyll content and malondialdehyde activity in Fenugreek plant were studied. Results were showed that magnesium shortage was increased of growth factors, that this increment meaningful in 0.05 of statistical level. In relation to calcium shortage, growth factors was decreased which in statistical level 0.05 showed meaningful difference than blank level, while in relation to leaf relative water content wasn’t meaningful difference than control level. In potassium shortage, shoot length, root and shoot fresh weight and leaf relative water content was meaningful increased, while in root and shoot dry weight was observed decrement that this decrement in statistical level of 0.05 was meaningful than blank level. In effect of phosphorous shortage, shoot length meaningful increased than blank level, while in root dry weight, shoot fresh weight and leaf relative water content decrement was observed that meaningful than the blank level (P<0.05). In nitrogen shortage, root length and it fresh weight and leaf relative water content was meaningful increased than blank, while in shoot fresh weight, root and shoot dry weight was meaningful decreased in statistical level of 0.05 than blank. Macronutrient elements shortage was caused decrement of chlorophyll a in Fenugreek leaves with nitrogen, potassium and phosphorous shortage than to blank level. In plants with calcium and magnesium shortage increase of chlorophyll a was observed. Also, macronutrient elements shortage was caused decrease of Malondialdehyde activity in root and shoot Fenugreek plant.