ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ)
Year:2021 | Volume:31 | Issue:3|Papers Count:10

Introduction: The antibacterial and antifungal effects of pomegranates peel and seeds are associated with the presence of phenolic compounds (flavonoids and tannin). These compounds accumulate in the skin and pomegranate juice and account for 92% of the antioxidant activity of pomegranates (Abid et al. 2017). Total tannins for pomegranate peel and pulp were reported to be 9. 73 and 0. 66%, respectively (Delavare et al. 2014). The different species of pomegranate have antibacterial and antifungal properties that can influence on a wide range of microorganisms (Carlton et al. 2000). Phenolic compounds in plants and foods by changing the gut microbial population can increase the amount of useful bacteria and reduce harmful bacteria (Katiyar 2002). It has been found that intestinal microflora plays a critical role in the health of the digestive tract and is dependent on the ration as the final source for metabolism of organic compounds. (Choct et al. 1996). To our knowledge, the effects of pomegranate by-products have been investigated on livestock and rumen function, however their effects on the intestinal microflora in ruminants have not been addressed. Therefore, due to the high concentration of tannins and phenolic compounds in the pomegranate by-products and their effect on intestinal microflora, the aim of this study was to investigate the effects of pomegranate pomace silage and pomegranate air-dried pomace on intestinal microflora in Mehraban fattening lambs. Materials and methods: Nine male lamb of Mehraban breed (mean weight of 27. 03± 3. 5 kg and mean age of 187. 8± 1. 4 d), were fed on three iso-nitrogenous and iso-caloric diets. Diets were balanced according to NRC (2007) recommendation including control diet, diet contain 27% pomegranate pulp silage (mixture of seed and pulp at equal ratio) and diet contain 31% air-dried pomegranate seed pulp. All three diets were fed for 60 d after 3 weeks for adaptation in individual pens with free access to salts lick and water. At the end of experiment all lambs were slaughtered and after than for enumeration of intestinal fluoromicrobes, one gram freshly digested specimens of ileum and cecum were collected. Samples were spread on the surface of agar medium. Colonies were counted by ophthalmic count and bacterial count was calculated as CFU/g (number of colonies per gram). The MRS agar and MacConkey (MC) medium were used for identification and enumeration of Lactobacillus spp and Escherichia coli respectively. All samples were incubated at 37º C for 24 hours. All colonies were enumerated and recorded as CFU/g of culture suspension. For confirmation of Escherichia coli detection on MacConkey agar medium, polymerase change reaction (PCR) was conducted as DNA extraction using commercial kit (Bioneer, Sout Korea), polymerase change reactions, and electrophoresis of PCR products. Detection of molecular bacteria was done using the primers of 23S rRNA gene PCR. The PCR process was initial denaturation at 94 º C for 2 minutes and totally 35 cycles, denaturation at 94 º C for 45 seconds, and extension at 72 º C for 2 minutes. All data was analyzed as a complete randomized design using SPSS software. Significant difference for means was considered at 0. 05 level of differences. Results and discussion: The results of this study showed that in MRS medium either in ilium or cecum, the number of lactobacillus bacteria in all groups were not statistically significant. The mean number of Escherichia coli decreased due to feeding of pomegranate by-products (P<0. 05), while the type of pomegranate by-product has not significant effect on number of Escherichia coli. The importance role of gut microflora is well recognized in GIT health, although population of gut microbes has been influenced by diet (Choct et al 1996). In contrast of useful effects of lactobacillus on GIT, Escherichia coli damages the intestine of animals and produces lipopolysaccharide (Munyaka et al. 2012). Tannins are considered as a toxin to microorganisms; these compounds in the soluble environment produce some stable complexes, mainly with protein and to a lesser extent with carbohydrates or some physiological ions elements such as iron and copper (Chung and Chou 1998). The pomegranate peel extract at different levels has antimicrobial effect against microorganisms such as Staphylococcus aureus, Escherichia coli, Candida tropicalis and Candida albicans (Ahmed et al. 2013). The phenolic materials in pomegranate fruit, are responsible for the antimicrobial properties of pomegranates (Seeram et al. 2006). In the present study, reduction of Escherichia coli population in lambs fed pomegranate by-products can be attributed to the adverse effect of phenolic substances in the pomegranate byproducts on Escherichia coli population. Several mechanisms have been introduced for antimicrobial properties of phenolic compounds in the pomegranate. Phenolic substances, with high molecular weight proteins, form complexes and by these complexes can react to the some cytoplasmic and membrane enzymes after absorption (Seeram et al. 2006). These complexes can also prevent cell surface receptors from attachment of harmful microorganisms (Cowan 1999). Phenolic compounds can react with the cellular proteins of microorganisms, alter cell wall structure and function (Hugo and Bloomfield 1971), reducing cell wall permeability and reducing substrate transport to cells (Goel et al. 2005). In addition, phenolic compounds can alter or denature some microbial enzymes, and also form complexes with certain nutrients and remove them from microorganisms (Hugo and Bloomfield 1971). The decline of Escherichia coli can also be attributed to the increase in the number of Lactobacillus; because by increasing the Lactobacillus and consequently increasing the production of lactic acid and creating an acidic environment, the population of Escherichia coli is reduced due to sensitivity of Escherichia coli to acidic environment (Hammer et al. 1999). Conclusion: Feeding of the pomegranate pomace silage and air-dried pomegranate pomace in fattening lambs, decreased the population of Escherichia coli in ileum and cecum, although lactobacillus bacteria was not affected by pomegranate by-products. It seems that the tannins and phenolic compounds present in the pomegranate can effect on Escherichia coli population in ileum and cecum.

Introduction: Livestock systems in developing countries located in tropical and sub-tropical regions are heavily dependent on the natural resources (i. e. pastures). In these countries, decreased pasture availability and quality during the dry season have negative effect on performance and health of dairy ruminants. In these conditions, energy intake does not meet energy requirements for body maintenance, fetal growth and milk production, which results in negative energy balance, and high adipose tissue mobilization. Studies have shown that the risk of metabolic disorders would considerably increase if adaptation to NEB fails, affecting performance as well as overall health and welfare of animal. Fat-tailed sheep, such as the Lori-Bakhtiari and Turkey-Qashqai breeds, are raised in semi-arid regions of Eastern and Southern Africa, Central Asia, as well as numerous countries in the Middle-East. The common characteristic of all fat-tailed sheep is the deposition of a substantial amount of fat in the tail. Fat-tailed sheep are known for being highly resilient to harsh environmental conditions such as those related to the dry season such as water scarcity and low quality pastures and feedstuffs. According to the literature, fat depots are differently regulated in fat-tailed sheep compared to other sheep breeds during periods of feed scarcity. However, most of these studies have been performed in sheep breeds used for meat production. Material and methods: The present study was carried out in the experimental farm of Yasouj University (Naregah, Yasouj, Iran). All animal procedures followed the ethical law on Animal Protection and were approved by the Committee of Animal Experiments (Yasouj University, Iran). All ewes used in this study were visually healthy and had no signs of diarrhea. The present study was carried out to investigate the negative effect of a reduced energy intake on the body weight and blood metabolites of non-pregnant fat-tailed ewes. In this experiment, 10 non-pregnant fat-tailed ewes (Lori-Bakhtiari and Turkey-Qashqai) with average age 3. 6 ± 0. 3 y and BW 49. 2 ± 3. 60 kg were used. During the trial, all animals were kept in individual pens (1. 2 × 1. 0 m 2 ) located in a closed barn. Each pen was equipped with a separate drink and feed container. Two weeks before the start of the experiment all animals were fed with a total mixed ration (TMR) diet formulated to fulfill 100% of the energy requirements recommended by the NRC (2007). Then, animals were randomly allotted into one of the two experimental groups, including control (n= 5) and the feed restriction (n=5) groups. Ewes of the control group had ad libitum access to the diet from wk 1 to wk 5 (end of experiment). The feed restriction group was fed with a diet equivalent to the 100%, 50%, 65%, 80%, and 100% of the energy content of the dry diet on wk1, wk2, wk3, wk4 and wk5, respectively. During the entire experimental period, TMR was provided to the animals twice a day (0800 and 1700). In addition, animals had free access to drinking water and mineral blocks throughout the entire experimental period. The individual feed intake was recorded daily by weighing the offered TMR and the ort in the next morning before feeding. The ewes were weighed weekly on the last day of each experimental week. During the entire experimental period, blood samples were collected weekly from the jugular vein using heparinized vacuum tubes (6 mL) at 0730 (before feeding). All blood samples were kept in wet ice and then centrifuged at 3, 500 × g for 15 min. The plasma was then aliquoted (1. 5 mL) and stored at-20° C. The plasma concentration of glucose, urea, creatinine, triglyceride, cholesterol, albumin, low density lipoprotein-cholesterol (LDL-C), high density lipoprotein– cholesterol (HDL-C), as well as the plasma activity of lactate dehydrogenase (LDH), glutamate pyruvate transaminase (GPT), and glutamic oxaloacetic transaminase (GOT) were determined using commercial kits (Pars Azmoun, Karaj, Iran), and NEFA and BHBA were measured by a RANDOX (Randox LTD, UK) commercial kit, and an automated analyzer (Mindray, B850, China), following manufacture’ s instructions. The concentration of very-low-density lipoprotein (VLDL) was determined using the following equation: VLDL (mg/dl) = TG × 0. 20. The data was tested for normal distribution using the UNIVARIATE procedure of SAS (SAS Institute Inc., Cary, NC, USA, 2002-2008, Release 9. 2). The data was evaluated using the MIXED procedure of SAS. The model included treatment (control and feed restriction), time (from wk 1 to wk 5), and the interaction (treatment × time) as fixed effects. The individual ewe was set as a repeated subject. Concentrations of metabolites were considered dependent variables. Significant differences were considered significant if P<0. 05, and a tendency if 0. 05

Introduction: To maximize the profitability of livestock industry decisions are based on individual animal yield and other factors such as overall health of animal as well as production and reproduction performance. To increase the revenue changes in replacement policies and breeding are essential (Nasr Esfahani 2018). Accordingly, in Iran issues such as feed price fluctuations and lack of coherent policies increase the economic risk of the dairy cow industry. To better make a decision on elimination or replacement of a cow, the producer shall compare the expected benefits of both keeping or replacing an individual animal. The most important objective of a livestock unit is to maximize the profit of the herd; one of the issues affecting this profit is the criteria and level of elimination (Rogers et al, 1988). If elimination and replacement is not optimal, namely the cows are eliminated sooner or later than the optimal time, the profitability of the herd will decreases. For determining the optimal elimination time point several biological and economic variables shall be concurrently taken into consideration (De Vries, 2006). The elimination decision must be based on predicted future incomes of the cow. Generally, keeping an animal in the herd for a longer time leads to more profit. The proper decision of optimal and reasonable elimination is made by comparing the present value of future liquidity flow (income and expenses) of the current livestock in the herd with the present value of the future liquidity flow of heifer as its replacement. Ultimately livestock with most current value will occupy the position (Groenendall et al. 2005). The application of dynamic programming in the animal sciences mostly is about animal replacement issues. The optimal policy at each stage represents best decision from that stage till the final stage. In this method, an expected value will be calculated for each of the situations that will happened. Furterhmore, the decision maker selects the best decision based on the calculated expected value and upcoming situations. For optimal replacement decision making in dairy herds, several dynamic programming models have been proposed by De Vries et al. (2006) and Van Arendonk et al. (1985). Cardoso et al. (1999) reported optimizing replacement and insemination policies in dairy cows by calculating income, costs and monthly probability of elimination. Material and methods: In this study, we estimated the biological parameters of the herd, including risk of forced elimination and the possibility of pregnancy in different lactation periods and different months after calving based on the data collected from industrial dairy farms (Ardabil city, Iran) between 2015 and 2018. Also, financial data of the herd was also obtained in the form of an economic questionnaire from the studied units and was developed by importing biological parameters and financial data into a bio economic model in Dairy Vip software. This software simulates the livestock over time and calculates the performance of herd based on optimal and non-optimal modes. The basis for optimal elimination decision is to minimize the cost of missed opportunity (cost of rejecting the best alternative when deciding). So that by negating the value of keeping livestock (obtained from the difference between the net present value of existing livestock and alternative heifers), optional removal will be done. The criteria of optional elimination in the studied herds were determined to reach the milk production of non-pregnant cows to less than 18 kg/day. Milk production was evaluated using daily milk production records and also fitting the incomplete gamma curve (Wood). The 21-day mean insemination rate and success insemination rate of cow were 49. 3% and 37%, respectively. By default, in Dairy Vip software, a livestock can be in the herd for 24 months maximum after calving. The risk of abortion from the second to the eighth month was 6. 24, 4. 16, 2. 08, 1. 11, 0. 45, 0. 19, and 0. 19%, respectively. Dynamic programming model was developed to determine the optimal replacement policy. The objective function in this study was to maximize the present value of net income from current cows and alternative heifers. In order to estimate the expected statistics under optimal policy, Markov chain simulation was used. Results and discussion: With the implementation of optimal policies, the annual elimination rate increased from 30. 11% to 43. 80%. However, the forced elimination rate slightly decreased )2. 3%(. The optimal removal rate can vary depending on the economic and biological conditions in the herd. With the implementation of optimal policies, pregnancy rate increased from 15. 31% to 18. 20% and increased by 2. 89%. Correspondingly, it can be concluded that the economic importance of increasing the pregnancy rate is more urgent in herds with weaker reproductive performance. Also, success rate of insemination dropped from 33. 9% in non-optimal mode to 37%, indicating higher likelihood of livestock pregnancy in different months. The 21-day insemination rate increased from 47. 6% to 49. 3%, indicating appropriate cow inoculation. milk production in dairy cows could be increased by reducing the pregnancy days. In this study pregnancy days decreased from 139 to 132 days by optimal policies leading to significantly higher milk production. Furthermore, open days (the interval from calving to the next gestation period) decreased from 167 to 161 days reducing the pregnancy days and subsequently increasing the pregnancy rate. Also, reducing the calving distance from 13. 6 to 13. 3 (0. 3 per month) resulted in higher annual milk production. Optimal policies compared with the nonoptimal policy resulted shorter lactation days thus significantly higher milk production (daily and annual) and performance (Table 3). Daily milk performance increased from 41. 4 to 44. 2 equaling to 3 Liters more milk per cow (annually 935 kg more milk). According to our observations, annual milk yield increased from 12, 548 to 13, 483 kg per cow via the implementation of optimal policies. Moreover, reducing the average lactation days resulted in increase in the daily and annual production of cows by 3 and, respectively. Conclusion: The most important goal of a livestock unit is maximizing the profit of the herd. One of the factors affecting profitability, is criteria and elimination rate. Implementing optimal policies is associated with increased livestock elimination rates and replacement costs and also increased feed costs. However, higher revenue from these policies can compensate the increased costs thus increasing the net profit per cow. One of the basic criteria in estimating the expected present value is to sort the cows in the herd based on future income and expenses, and according to these values, the decision is made to keep or eliminate the cows. So, regardless of these values, cows may be eliminated sooner or later than the optimal time, which reduces the profitability of the herd.

Introduction: Supplementing the feed with microbial products or probiotics have shown to improve animal performance and enhance the growth of young ruminants by maintaining a microbial balance in the intestinal flora thus preventing gastrointestinal infections (Noori et al. 2016). The use of bacterial probiotics in milk substitute of suckling animals has improved the feed conversion ratio (Noori et al. 2016) and increased daily weight gain (Kawakami et al. 2010). Probiotic-containing feed improves animal weight gain presumably via improvements in microbial ecology (Lascano et al. 2009), increased nutrient uptake (Khutia and Chayyaderi 2002) and improved feed conversion ratio. The effects of bacterial probiotic use on different performance, health status, and blood counts have been reported, and differences in results may be due to various factors (e. g., type of probiotic, type of feed, level of management, method of probiotic use, and environmental conditions) (Agarvall 2002). The results of a study showed that the production of growth factors (organic acids, B vitamins and amino acids), creating anaerobic conditions and increasing the growth of cellulosic bacteria and lactate consumption are among the mechanisms of probiotics in increasing the digestibility of food nutrients (Riedel et al. 2010). Considering the advantages of using probiotics in feeding young ruminants and suckling animals, which improved growth performance and also reduced the use of antibiotics, and most importantly, the growth and development of healthy and prolific lambs that can replace productive ewes and adult rams in the herd in the future and ensure the economic future and health of the herd. However, no study has yet tested the use of oral probiotics in lambs and lactating Bakhtiari ewes. In this study we aim to investigate the probiotic effects of protexin on blood hematological parameters of lactating Bakhtiari lambs. Materials and methods: In this experiment, 28 ewes with one to multiparous were used. The ewes were divided into two groups. One group (14 head) received no probiotics and the other group (14 head) received 2% probiotics solution (v/w) (one gram of probiotic dissolved in five mL of water) per day through oral and syringe for the duration of one month until parturition. Probiotics were discontinued at parturition. The lambs born of these ewes were 28 (male and female). Treatments included 1) control treatment (non-reception of probiotics by lambs and ewes), 2) 1 g of probiotic by ewes, 3) 0. 2 g of probiotic by lambs 4) 0. 2 g of probiotics by lambs and 1 g of probiotics by ewes. The lambs were weighed weekly at birth and up to five weeks after birth. In order to measure hematologic and hematological parameters on 3, 14 and 21 days after the morning and by applying food restriction for 2 to 4 hours, blood was taken through the vein from all lambs. Blood samples were taken at 3, 14 and 21 days to measure hematological and blood parameters. Hematological parameters included eosinophils, lymphocytes, monocytes, neutrophils, hematocrits, and white blood cells. Blood parameters included glucose, albumin, total protein, globulin, triglycerides, alkaline phosphatase, gamma glutamine transferase, acute phase proteins, glutamic oxalacetic transaminase, and iron oxalate, copper and iron, calcium, iron, phosphorus, and zinc. Hematology supplements and blood suppositories were determined using an autoanalyzer (model BT 1500, made in Italy). Results and discussion: The effect of protexin probiotic on body weight in lambs was not significant (P>0. 05). Consistent with our result, the use of probiotic supplementation in lamb feeding during the experiment did not have any significant effect on daily weight gain in another study (Baranowski et al. 2007). The effectiveness of compounds such as probiotics, which have different microorganisms, varies, and depends on the composition of the animal's diet and nutritional needs, and may be ineffective with the slightest change. Therefore, animal feed management includes feed supply (completely mixed feed, separate forage and concentrate feed), number of feed times and physical shape of feed, chemical composition of feed including forage to concentrate ratio, dietary nutrient percentage, dietary effective fiber percentage and forage type. The concentrate used in this study can be considered as a possible cause of differences in results. The effect of treatment on hematological data including eosinophil, lymphocyte, monocyte, neutrophil, hematocrit, and white blood cell concentration in lambs was not significant (P>0. 05). Hematological parameters are important in determining the functional status of animals. Our results agreed with the study reporting modulatory effects of biofeedback probiotics in the last month of gestation and by the end of lactation on the lambs' red blood cell counts, neutrophils, lymphocytes, basophils, and eosinophil percentages (start and end of experiment). Regarding the blood parameters, the effect of treatment on the concentration of albumin, alkaline phosphatase, total protein, triglyceride, globulin, gamma glutamine transferase, glutamic oxalacetic transaminase, glucose, albumin to globulin ratio, and acute phase proteins was not significant (P>0. 05). Probiotic supplementation had insignificant effect on total protein, albumin, globulin, and glucose concentrations of lambs in the pre-weaning period (Salim et al. 2017). Also, the addition of protexin probiotics had insignificant effect on blood minerals (calcium, zinc, iron, copper) in lambs (P>0. 05). Consistent with our results, Zovitis probiotic supplement had no significant effect on blood phosphorus and calcium concentrations (Dimova et al. 2013). Conclusion: Probiotic supplementation of protexin had no significant effect on lamb weight, hematological parameters, and blood parameters. This indicates that this probiotic had no positive effect on lambs. There is a need for more research and more levels of this probiotic in the lambs of Lori Bakhtiari.

Introduction: As is already known, urea transport across the ruminant wall is mediated via urea transporters in the epithelium membrane (Stewart et al. 2005). Facilitative urea transporters are derived from the UT-A and UT-B genes. UT-B mRNA or protein expressions have been characterized in the rumen epithelium (Ludden et al. 2009; Marini and Van Amburgh 2003; Marini et al. 2004; Stewart et al. 2005). Gene transcript abundance for UT-B is significantly correlated with the ruminal serosal to mucosal urea fluxes. However, the mechanism by which the increased gene expression occurred is unclear (Jin et al. 2018). Transcriptome analysis has been used to analyze the rumen epithelium metabolic pathway changes under various conditions (Baldwin et al 2012; Dionissopoulos et al 2014; Naeem et al 2014). The regulation of these urea transport mechanisms across the rumen wall may be reavealed through this approach. Material and methods: Literature searches were performed through various databases (Google Scholar, PubMed, Medline, Science Direct, Scopus, Web of Science). The keywords used to search relevant studies included: carbohydrate, dietary, protein and urea transporter. Based on the selection criteria, only 11 articles were included in the database for the final analysis. The response variables extracted for the meta-analysis included pH, VFAS, N-NH3 and UT-B expression. Meta-analysis was carried out using the Comprehensive Meta-Analysis package, version 3. The effect sizes of across studies were calculated with fixed and random effect models. The presence of true heterogeneity among studies was identified with Cochran’ s Q– tests and quantification of the degree of heterogeneity was done with the I 2 index. Possible publication bias was evaluated with funnel plot and statistical tests (Vesterinen et al. 2014). Results and discussion: The results of current meta-analysis showed that different levels of CP in diet, has a positive effect on ruminal N-NH3 concentration and UT-B expression (<0. 05). However, different levels of dietary CP had insignificant effects on ruminal pH and VFAS concentration. The values of I 2 and Q for ruminal N-NH3 concentration and UT-B expression effect size indicated high and moderate heterogeneity, respectively. Different levels of NFC in diet increased ruminal VFAS concentration and UT-B expression (<0. 05). Different levels of dietary NFC decreased ruminal pH (<0. 05). Ruminal N-NH3 concentration was not influenced by different levels of dietary NFC. The values of I 2 and Q for pH and UT-B expression effect size indicated high heterogeneity. Conclusion: According to the results of current meta-analysis, both dietary CP and NFC increase UT-B expression. It seems however that the NFC have a greater effect on increasing UT-B expression than CP.

Introduction: In the small intestine, villi height, crypt depth, epithelial tissue thickness, and the ratio of villi height to crypt depth play a significant role in the final stages of digestion and absorption of nutrients from the gastrointestinal tract (Strusiń ska et al. ., 2009, Wang et al. 2009). The special shape of the villi and the presence of small intestinal micropores increase the level of intestinal absorption and have a direct effect on their efficiency and function (Kreikemeier et al. 1991). The presence and absorption of volatile fatty acids are the keys to developing the tissue structure of the gastrointestinal tract (Attaix and Meslin 1991 and Vi et al. 2004). The effect of volatile fatty acids on stimulating cell proliferation and growth of gastrointestinal tissue is not limited to the reticulum and rumen, but also affects the small intestinal villi. (Wang et al. 2009). Feeding animals with high concentration diets could cause metabolic problems including acidosis (Aschenbach et al. 2002). Its results are included the death of gram-negative bacteria and the release of endotoxins (lipopolysaccharides) into the bloodstream from the damaged rumen wall, activating inflammatory mediators, and affecting the productive function of animals (Garcia Diaz et al. 2018). The possible effects of microbial additives on the gastrointestinal tract are through the regulation of ruminal pH and the immune system, which are useful for improving digestion, function, and animal health (Ding et al. 2008 and Silberberg et al. 2013). The use of yeast and other biologically active compounds such as lactic acid consuming bacteria compared to chemical buffers could be effective in reducing inflammation caused by acidosis. (Aschenbachet al. 2019). Material and methods: Twenty-four Arabic male lambs with Four months old and initial body weight of 23. 9± 3. 15 kg were used in a completely randomized design with three treatments and eight replicates. The trial period consisted of 77 days (11 weeks) including 14 days of habituation period and 63 days (9 weeks) of the recording period. The lambs were randomly assigned to one of three treatments: 1-control (no additive) 2-control + Sodium bicarbonate (1% daily diet in two meals) 3control + Megasphaera elsdenii and Saccharomyces cerevisiae (bacterium-yeast). The amount of Megasphaera elsdenii was 3 ml per animal (4. 5 × 108 cfu / ml) plus 2 g of Saccharomyces cerevisiae (DFM) fed to the animals daily in the morning (Sedighi and Alipour 2019). The diets were adjusted using the Small Ruminants Nutrition Requirements (NRC 2007). The lambs were fed a fully mixed (TMR) ratio of 30% forage and 70% concentrate at two meals (8 and 16 hours) with free access to water. For the histological trial of the intestines, fragments were separated from the middle part of the three regions of the duodenum, jejunum, and ileum, and the middle part of the large intestine and each part were emptied with formalin rinsing, Then the two ends of each piece were closed and neutral formalin 10 % was injected into them, and separately in containers with lid containing formalin 10 % were placed. Micrometric studies of different parts of the small intestine: Villus height, crypt depth, villus width, and thickness of tunica muscularis were examined histomorphometrically. Results and discussion: villi height, crypt depth, and villi surface of the duodenum in bacterium-yeast treatment were higher than other treatments (P<0. 05). The thickness of tunica muscularis in the buffer treatment was higher than other treatments (P<0. 05). The width of duodenum villi in the buffer and bacterium-yeast treatments was higher than the control treatment and was significant in the bacterium-yeast treatment than the control treatment (P<0. 05). No significant difference was observed between jejunum tissue indexes in the treatments. The height of ileum villi in buffer and bacterium-yeast treatments was higher than the control treatment and was significant in the bacterium-yeast treatment than the control treatment (P<0. 05). Crypt depth and villi surface of ileum in bacterium-yeast treatment was higher than other treatments (P<0. 05). In buffer and bacterium-yeast treatment due to pH adjustment and increased activity of fiber degrading bacteria which produce more acetate (Lechartier and Peyraud 2011 and Malekkhahi et al. 2016) The dimensions of the villi of the small intestine increased. In Histopathological investigation of the duodenum in all three treatments of control, buffer, and bacterium-yeast, infiltration of mononuclear inflammatory cells in the parenchymal mucosa was observed. In terms of lesion severity, the highest involvement was in the mucosa of the control treatment and then in the sodium bicarbonate buffer treatment and was less in the bacterium-yeast treatment than the other two treatments. In the bacterium-yeast treatment, the penetration of mononuclear inflammatory cells in the parenchyma of jejunal mucosa was much less than control and buffer treatments. In bacterium-yeast treatment, the frequency of Peyer's patches in the ileum is reduced compared to other treatments, which indicates a reduction in tissue inflammation. Histopathological investigation of the large intestine showed the infiltration of mononuclear inflammatory cells in the control treatment. The penetration of mononuclear inflammatory cells in this part was less than that of the small intestine. The severity of tissue lesions in the buffer and bacterium-yeast treatments was lower than the control treatment. In bacterium-yeast treatment, the penetration of mononuclear inflammatory cells was greatly reduced. Conclusion: Compared to the control, the use of pH regulators in terms of histopathology reduce tissue damage in the small intestine and large intestine. The use of pH regulators, especially the bacterium-yeast composition, has led to the development of intestinal mucosal indices such as villi height and crypt depth. In addition, the destructive effect of acidosis, such as the penetration of inflammatory cells and necrosis, which was the result of increased consumption of high concentrates on digestive tissues; Has been reduced. Therefore, the use of pH regulators, especially bacterium-yeast, improve the tissue structure of the small intestine and they are reduced tissue damage in the small and large intestines.

Introduction: In order to take advantage of breeding programs and productivity of poultry and other domesticated animals, it is essential to assess genetic variability and study the strategies to preserve genetic diversity. The Japanese quail is widely used as a model for animal research purposes in laboratory studies because of its small body size, short intergeneration interval, high growth rate, production of more eggs and meat. Although, Japanese quail has various benefits as a laboratory bird, but its genome sequence is not accessible now. The genome sequence of Japanese quail will deliver important genomic resources to accelerate different studies and to authenticate divergent lines of Japanese quail. Growth is a complex physiological pathway that occurs from fertilization until maturity in birds. Insulin-like growth factor I (IGF-I) gene is one of the most important candidate genes in different species which can affect the performance traits because of its function in metabolism and growth. IGF-I is a 70 amino acid polypeptide hormone with endocrine, paracrine, and autocrine effects. It has been reported that there is association of genetic polymorphisms of the IGF-I gene with growth traits in the poultry. There are relatively few reported studies about the relation between genetic markers of the IGF-I gene and body weights in quails. The objective of this study was undertaken to identify polymorphisms of the IGF-I gene using PCR single-strand conformational polymorphism (PCR– SSCP) analysis and to evaluate association of these polymorphisms with body weight in the Japanese quail. Material and methods: For doing this research, body weight of the 110 quails under study (46 males and 64 females) in different ages were collected. All data were collected from the Natural Resources Research Center of East Azerbaijan. The data included the identification of the animal, year of birth, sex, body weights at 15, 30, 45 and 60 days. Genomic DNA was extracted from 10 μ l of blood in presence of Pronase (Bailes et al. 2007) and stored in EDTA-coated tubes and placed immediately inside an ice box and transferred to the laboratory. The samples were stored at-20 until DNA has been extracted. The quality and quantity of extracted DNA were measured by %0/8 agarose gel electrophoresis. PCR was done for amplifying a fragment in size of 465 bp of IGF-I gene. The PCR primers for the IGF1 gene were designed based on GenBank. PCR primers of IGF-I gene was mentioned in Table 1. The PCRs were carried out in 25 μ l volumes containing 1 unit Taq DNA Polymerase, reaction buffer 1X (Sina Gene, Tehran, Iran), 1/5 mM MgCl2, 0/2 μ M each of dNTPs, 10 μ M of each primer (Sina Gene, Tehran, Iran) and 30 ng of genomic extracted DNA as template. PCR was performed using the T-professional thermal cycler. The thermal profile consisted of 3 min at 95° C, followed by 35 cycles of 40 s at 95° C, 30 s at 58° C and 40 s at 72° C, with a final extension of 5 min at 72° C. Amplification was carried out in Mastercycler (Bailes et al. 2007). PCR products were detected by electrophoresis on %1/5 agarose gel containing ethidium bromide and were visualized in a gel documentation system with a UV transilluminator. PCR products were mixed with 20 μ l of denaturing loading dye [95% deionized formamide, %0/35 xylene cyanol, %0/25 bromophenol blue and 10 mM EDTA] in a total volume of 10 μ l. The mixture was denatured at 95° C for 15 min and was snap chilled on ice. The electrophoresis was performed in 0. 5X TBE buffer (Tris 100 mM, boric acid 9 mM, EDTA 1 mM) at room temperature (18° C) and constant 110 V for 16 h. Polyacrylamide gels were stained with silver according to the protocol described (Benbouza et al. 2006). A statistical model included the mean of population, fixed effect of the sex, random effect of the genotype patterns and residual random term was done by using GLM of SAS software to find the association between the SSCP genotype patterns of PCR products with the body weights. Significant differences among means of different genotypes were calculated using Duncan method in the GLM program and P-values of 0. 05 were considered statistically significant. Results and discussion: The investigation of candidate genes is one of the foremost techniques to reveal whether definite genes are associated with the economic traits in animals. We successfully amplified the exon 4 of the IGF-I gene. All extracted DNAs from quail blood samples yielded a specific single band PCR product without any nonspecific band. Therefore, the PCR products were directly used for SSCP analysis. The results of SSCP showed that this population was polymorphic at the studied loci and three different genotypes with frequencies of 7. 27%, 50. 91% and 41. 82%, respectively were observed in the examined quails (Figure 3). The results indicated that the exon-4 of IGF-1 gene is polymorph and there was a significant difference (P<0. 05) between the genotype patterns and body weight on 30 days. However, for association of gender effect on body weight, there was a significant difference (P<0. 05) in the age of 60 days. Also the average daily gain in females is more than males in different ages. Conclusion: The goal of this study was to determine genetic polymorphism of IGF-I gene in Japanese quail. According to this research, the selected locus for investigating of IGF-I gene in quail can be considered as a locus that effects on body weight and growth rate of quails. It can be also measured as the factor that cause of difference of body weight in different periods.

Introduction: Understanding the genetic control of growth traits is essential for effective poultry breeding poultry. One way to identify new loci and confirm existing QTL is through genome-wide association analysis (GWAA) (Wang et al., 2011). In addition, identifying loci with large effects on economically important traits, has been one of the important goal to poultry breeding. QTL assisted selection and genomic regions affecting the production traits have been considered to increase the efficiency of selection and improve production performance (Seabury et al., 2017). Genome wide association studies typically focus on genetic markers with the strongest evidence of association. However, single markers often explain only a small component of the genetic variance and hence offer a limited understanding of the trait under study. A solution to tackle the aforementioned problems, and expand understanding of the genetic background of complex traits, is to move up the analysis from the SNP to the gene and gene-set levels (Peñ agaricano et al., 2013). In a gene-set analysis, a group of related genes that harbor significant SNP previously identified in GWAS, is tested for over-representation in a specific pathway. Material and methods: The aim of the this study was to genome wide association studies (GWAS) based on Gene set enrichment analysis for identifying the loci associated with related to body weight and shank length and diameter traits in advanced intercross line (AIL) using the high-confidence SNPs that enable us to study 161376 SNP markers simultaneously. For this purpose, the 599 advanced intercross line and 161376 markers were performed with mixed linear model (MLM) approach was used for the GWAS of the F9 generation, as implemented in the GCTA package (v1. 92) (Yang et al., 2011) and no any correction to adjust the error rate. The gene set analysis consisted of three different steps: (1) the assignment of SNPs to genes, (2) the assignment of genes to functional categories, and (3) the association analysis between each functional category and the phenotype of interest. In brief, for each trait, nominal P-values < 0. 005 from the GWAS analyses were used to identify significant SNP. Using the biomaRt R package, the SNP were assigned to genes if they were within the genomic sequence of the gene or within a flanking region of 15 kb up-and downstream of the gene, to include SNP located in regulatory regions. For the assignment of the genes to functional categories, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway databases were used. The GO database designates biological descriptors to genes based on attributes of their encoded products and it is further partitioned into 3 components: biological process, molecular function, and cellular component. The KEGG pathway database contains metabolic and regulatory pathways, representing the actual knowledge on molecular interactions and reaction networks. Finally, a Fisher’ s exact test was performed to test for overrepresentation of the significant genes for each gene-set. The gene enrichment analysis was performed with the goseq R package. In the next step, a bioinformatics analysis was implemented to identify the biological pathways performed in BioMart, Panther, DAVID and GeneCards databases. Results and discussion: Gene set enrichment analysis has proven to be a great complement of genome-wide association analysis (Abdalla et al., 2016). Among available gene set databases, GO is probably the most popular, whereas KEGG is a relatively new tool that is gaining ground in livestock genomics (Morota et al., 2015, 2016). We had hypothesized that the use of gene set information could improve prediction. However, neither of the gene set SNP classes outperformed the standard whole-genome approach. Gene sets have been primarily developed using data from model organisms, such as mice and flies, so it is possible that some of the genes included in these terms are irrelevant for meat production. It is likely that a better understanding of the biology underlying meat production specifically, plus an advance in the annotation of the chicken genome, can provide new opportunities for predicting production using gene set information. Eleven SNP markers on chromosomes 1, 2, 4, 5, 7, 8, 10, 11, and 27 located in MSTN, CAPN3, PNPLA3, ANXA2, IGF1, LDB2, LEPR, FN1, TMEM135, MC4R, EDN1, and ADAMTS18 genes were identified. Some of the genes found to be consistent with some previous studies. Those seem to be involved in biological pathways related to muscle skeletal growth, energy metabolism and bone growth and development. According to pathway analysis, 19 pathways from gene ontology and KEGG pathway were associated with the body weight, shank length and diameter trait (P˂ 0. 05). Among those pathways, the regulation of muscle organ development, regulation of cell growth and anatomical structure homeostasis biological pathway have important roles in the growth and skeletal muscle development. Also, the anatomical structure formation involved in morphogenesis, positive regulation of ossification and calcium signaling pathway presumably has significant association with body weight and shank length as well as diameter traits. Some of these regulatory regions, such as enhancers, are located far from the genes. Therefore, although the gene might be part of the analysis, the relevant variant would probably not be included in the gene set SNP class. Finally, linkage disequilibrium interferes with the use of biological information in prediction because irrelevant regions (regions without any biological role) capture part of the information encoded in relevant regions, causing both regions to exhibit similar predictive abilities. The use of very high density SNP data or even whole genome sequence data could alleviate some of these issues. Finally, it is worth noting that our gene-set enrichment analysis was conducted using a panel of SNP obtained from a single marker regression GWAS, which relies on a simplified theory of the genomic background of traits, without considering for instance the joint effect of SNP. Hence, other approaches (e. g., GWAS exploring SNP by SNP interactions) might provide a better basis for biological pathway analysis. Conclusion: Our observations agreed with the previous results from GWAS of body weight, shank length and diameter traits. Moreover, additional regions in the chicken genome associated with economically important traits were revealed. Our findings would contribute to a better understanding of the genetic control of growth traits in broiler chicken accelerating the genetic progress in poultry breeding programs.

Introduction: Long-term storage of semen is essential for achieving the benefits of artificial insemination (Tuncer et al. 2010). This is carried out by sperm cryopreservation, which stops the sperm metabolic activities, allowing long storage (Bailey et al. 2000). This process affects the sperm quality (Wang et al. 1991) by introducing mechanical and chemical damage, production of reactive oxygen species, oxidative stress, and reducing antioxidant activity (cysteamine is an aminothiol antioxidant as an effective scavenger). Cysteamine is known to have been reported in some studies to improve the freezing of ram sperm (Bucak et al. 2007). The aim of the present study was to determine the antioxidant effects of cysteamine on the functional parameters of cysteamine in Lake Extender based on soybean lecithin. Material and methods This study was carried out in University of Tabriz research station. For this purpose, 15 adult roosters (25 weeks old) were used. Sperm collection was done by dorsal-abdominal massage. The roosters were habituated for one month and sperm collection was performed twice a week. First, sperm were examined for volume, concentration and color, and only samples with volume of 0. 2 to 0. 7 mL and motility ≥ 80% and concentration above 3×10 9 were used. To eliminate the individual effects, the confirmed samples were pooled. Four levels containing control, 0. 15, 0. 30, and 0. 45 μ M cysteamine were then added to Lake Extender containing (one part of semen and four of extender). The cooling process was carried out in two steps. The samples were adjusted to 4° C for two hours. Samples were transferred to the refrigerator for one more hour, then they were drawn into 0. 25 mL straws, placed 4 cm above nitrogen vapor for 7 min, and immersed in liquid nitrogen. They were stored in liquid nitrogen until further analysis. For assessment, the cryopreserved straws were thawed in a water bath at 37 ° C for 30 s. The motility parameters were evaluated using CASA, viability by Eosin-Nigrosin staining, membrane integrity by Host tests, sperm abnormality by Hancock test and lipid peroxidation by MDA. Results and discussion: Based on the results (Table 2), addition of 30 and 0. 45 μ M significantly increased total motility and 0. 30 μ M level improved progressive motility, VAP, VSL and VCL parameters (P <0. 05). The results show that the addition of cysteamine amino acid improves sperm quality. Addition of 0. 30 μ M level significantly increased viability and plasma membrane integrity of rooster sperm (P <0. 05) while significantly decreasing malondialdehyde compared with the control group (P <0. 05). The addition of cysteamine insignificantly reduced sperm abnormality. The aim of this study was to reduce the production of ROS and the rate of lipid peroxidation while improving membrane health and survival. Bird sperm has an advanced antioxidant system that includes the antioxidants glutathione, peroxidase, and superoxide dismutase (Surai 1999). Adding antioxidants can control ROS production (Amini et al. 2015). Antioxidants in different species and at different doses have shown different results (Nazari and Daghigh kia 2020). Addition of cysteamine to rooster spermatozoa during freezing and thawing significantly increased motility and survival parameters, which agreed with the results of Bucak et al. (2007). Najafi et al. (2014) indicated that cysteamine at 6 mM improved viability and reduced lipid peroxidation (malondialdehyde concentration). cysteamine improved membrane functionality significantly, except at 8 mM. n. This was probably due to the high dose of diluent used. Our results did not agree with the study of Thananurak et al. (2019) who reported the negative effect of adding levels of 0. 001, 0. 002 and 0. 004 µ M on motility and viability. Low levels of cysteamine induce cysteine to enter the cell, producing glutathione as an intracellular antioxidant. Cysteamine at high doses produces large amounts of hydrogen peroxide, causing oxidative stress and reducing glutathione peroxidase activity (Besouw et al. 2013). According to report Partyka et al. (2013) cysteine increases motility and viability of rooster sperm. Glutathione peroxidase is one of the enzymes with peroxidative activity that plays an important role in sweeping high oxidations and protecting the cell from oxidative stress. Glutathione can also restore the oxidized vitamins E and C and restore them to the original antioxidant structure (Almasi et al. 2014). Cysteamine as a glutathione synthase can play a major role in reducing free radicals. In the present study, the amino acid cysteamine decreased malondialdehyde concentration and increased plasma membrane integrity and normal sperm during the freezing-thawing process. This agreed with the result of Najafi et al. (2014) reporting that addition of 6 mM reduced the concentration of malondialdehyde in ram semen compared with the control group. Not only does cysteamine improve sperm quality after freeze-thawing, but also it increases sperm resistance during artificial insemination in the reproductive female tract (Najafi et al. 2014). In the present study, cysteamine did not significantly decrease the percentage of abnormal sperm compared with the control group. Conclusion According to the results of this study, adding amino acid cysteamine to the diluted sample of semen can protect sperm from oxidative stress and reduce lipid peroxidation. Levels of 0. 30 and 0. 45 µ M increase the mobility. Also, the level of 0. 30 µ M increases the viability and health of the sperm membrane and reduces the amount of malondialdehyde in the rooster's semen.

Introduction: Compensatory growth is actually a physiological process in which the animal's potential, after a period of dietary restriction, can be exploited to increase growth and growth efficiency during the free feeding period (Hornick et al 2000). Compensatory growth is widely used today in various countries. Another specific management approach (short-term strategies) in lamb fattening is appropriate weight at the beginning of fattening (Santos Silva et al 2002). The benefits of applying a compensatory growth mechanism during the breeding period can be improved feed efficiency during the compensatory growth period (Abouhif et al 2013 and Sami et al 2016), reduced feed cost throughout the breeding period, also improved weight gain during the breeding period (Clark et al 2007; Abuhif et a l 2015) and reduced energy maintenance needs (Kamalzadeh et al 2009; Shadnosh et al 2011). Considering the feedlot of 320, 000 fattening lambs in Lorestan province per year and the possibility of using feed restriction to promote the use of compensatory growth in lamb fattening, the project was carried out aims to investigate the possibility of process changing the growth rate, fattening and physical and chemical composition of carcass lambs Lori breed by short-term strategies of feed restriction. This study was conducted to investigate the effect of different weight start of feed restriction on growth performance and carcass lambs traits Lori breed lambs. Material and methods: Fifty four male Lori lambs with a mean live weight of 26 kg were selected for the experiment. Treatments were consisted of 6 feed restriction treatments (60, 80%) and ad libitum(fattening diet without restriction) for two weights (30 and 35 kg). At the beginning of expriment, 54 experimental lambs were fed the same diet. After a 14 day pre-feed restriction period, a group of 27 individuals that had reached an average weight of 30 kg were separated from the rest and subjected to feed restraint treatments. The remaining 27 lambs were fed the former diet for 35 days until reaching a weight of 35 kg. Upon reaching this group, an average of 35 kg dietary restriction was applied. The feed restriction period for both groups was 30 and 35 kg for 35 days. Then the 30 kg group for 49 days and the 35 kg group for 28 days until the end of the fattening period were re-fed the control diet (fattening ration without restriction and ad libitum). At the end of experiment, daily weight gain, final weight, feed conversion ratio, feed efficiency and feed intake were calculated and then three lambs were slathered from each treatment. Carcass characteristics were calculated with the cost of production per kg of weight gain, carcass and lean meat. Analysis were done by repeated measurements basis on a completely randomized design with a mixed model procedure. Comparison of mean treatments was performed by Tukey test. Results and discussion: The results of Table 2 showed that the effect of the experimental diets had no significant effect on all the traits of lambs weighing 30 and 35 kg over the pre-feed restriction period. Results showed that there were significant difference between the final weight of lambs at the end of feed restriction period (P<0. 05). At the end of the re-feeding, no significant differences were observed among the control treatment (30 and 35kg) with 60 and 80% feed restriction groups. In the present study, at the beginning of the re-feeding period in the respective treatments, the daily weight gain in the feed restriction treatments was significantly higher than the control groups at 30 and 35 kg. There were significant difference among intake dry matter of lambs at the end of feed restriction period (P<0. 05). At the end of compensatory growth, there was observed no significant difference between control and restriction groups. During the re-feeding period, feed conversion ratio was lower in feed restriction treatments compared to control treatment (30 and 35 kg). Compensated feeding reduced FCR in lambs with prior feed restriction compared to control. The results showed that feed efficiency and feed intake needed to maintain live weight are affected by the animal feeding program. Results of physical composition of carcass during re-feeding showed that the average of most carcass traits in the control treatment (30 and 35kg) and 80% of feed restriction treatment for 30kg lambs had no significant difference and were higher than other groups. Also in the compensatory growth experiment, there was no significant difference between the number of empty body weight, hot carcass and cold carcass weight sa well as dressing percentage due to no difference in the final live weight between the 30 and 35 kg control groups with 80% feed intake. Another study in other ruminants was consistent with feed restriction and compensatory growth. Meanwhile, the fat tail weight and percentage and total carcass fat were higher in the control treatment (30 and 35kg) than in all control groups. The results showed that the percentage of carcass lean meat in the compensatory groups was higher than the control treatments. This study showed that lambs with 80% feed restriction for 30kg lambs due to higher weight gain and FCR were more favorable in terms of fattening condition than control lambs who consumed feed during whole period. Also, this treatment was the total carcass fat percentage lower and lean meat higher and ultimately, the production costs per kg carcass and lean meat were lower than other treatments.