The aim of this study was to examine the relationship of emotional intelligence and mood with self-efficiency and performance in handball players of Iran league 1. The statistical population consisted of all handball players (n=115) (9 teams) who participated in matches of league 1 in Golestan province (March 2010).95 athletes (M=21.46, SD=2.31) were randomly selected as the sample. Emotional Intelligence Scale (EIS) of Cyberia Shrink that consisted of 5 subscales (self-awareness, self management, self-motivation, empathy, social skills) on a 5-point Likert Scale anchored by “not at all” (0) to “extremely” (4) was used to evaluate emotional intelligence and mood of athletes. Brunel Mood Scale with 6 subscales (anger, confusion, depression, fatigue, tension, and vigor) was applied to measure game vigor. Also, Feltz Self-Efficiency Questionnaire was used to evaluate athletes’ ability to achieve the mentioned aims. K-S test showed that the data distribution was normal (P˃0.05). Descriptive statistics (mean and standard deviation), multivariate analysis of variance (ANOVA test) and post hoc tests were conducted to determine those subscales influencing self-efficiency and performance of handball players as well as Pearson correlation coefficient (P£0.05). The findings showed a significant relationship of mood with self-efficiency (F=5.29, P<0.000) and performance (F=3.46, P<0.00) in high-ranking, medium and low-ranking teams. Although there was no significant relationship between self-awareness (sig=0.23) and empathy (sig=0.16) with performance, the relationship between emotional intelligence and performance (F=6.28, P<0.03) was significant.