The aim of this study was to assess the effect of resistance training on apelin and inflammatory markers (Interleulin-6 and C-reactive protein) in sedentary obese men. In this quasi-experimental study, 20 men aged between 48 and 60 (BMI³30 kg/m2) were randomly selected and divided into control (n=10), and resistance training (n=10) groups. The training program consisted of 12 weeks of resistance training, 3 sessions a week, 3 sets with 10 to 12 repetitions per session with the intensity of 50-75% of one repetition maximum and 60-90 seconds of rest. The training included bench press, axillary rowing, behind the neck barbell shoulder press, leg press, front leg extension and behind leg curl. Before and 48 hours after 12 weeks of resistance training, blood samples were collected to measure apelin, Interleukin-6 (IL-6), Creactive protein (CRP) and insulin resistance index. Paired sample t test and independent t test were used for data analysis (P<0.05) using SPSS20 software. Results revealed that 12 weeks of resistance training significantly reduced IL-6 (P=0.036), CRP (P=0.011), weight (P=0.029), body mass index (P=0.021) and fat percent (P=0.005). Apelin levels (P=0.06) and insulin resistance index (P=0.089) decreased in the experimental group but it was not statistically significant. Generally, 12 weeks of resistance training, despite significant decreases in inflammatory markers, did not significantly decrease apelin levels. It seems that the observed changes in inflammatory markers were due to reduced body fat mass and anti-inflammatory nature of resistance training.