The purpose of this study was comparing mental toughness of athlete men and women in contact and non-contact sports at different levels of skill. In this regard, after an pilot study, 300 athlete men and women in three contact sports including Wushu, Kickboxing and Taekwondo as well as three noncontact sports including Badminton, Tennis and Squash in three levels of elite, non-elite and novice, were purposefully selected. In order to collect the data, sports mental toughness questionnaire (SMTQ) developed by Golby & Sheard (2009) was employed. The date were analyzed based on multi- Variable analysis test (MANOVA) and significant level of p<0.017. The results demonstrated that each of the main factors such as gender, sports type, and different levels of skill have significant effects on the amount of athletes' mental toughness in a way that men's mental toughness is significantly more than women's mental toughness, mental toughness of contact sports is more than more than non-contact sports, and mental toughness of elite athletes was more than non-elite and novice. On the contrary, four interaction effects involving the interaction of gender and sports type, gender and different levels of skill, types of sport and different levels of skill, and finally the interaction of gender, sports type and different levels of skill, had no significant effect. Different factors such as time condition, competition experience, age, activity level, psychological interventions, the nature of sport field and individual differences play roles in forming and developing mental toughness which require more studies.