Objectives: The purpose of this study is to evaluate the relationship between university atmosphere and its influence on Isfahan university students' educational motivation.Method: Data collection method is cross-correlation. The population of this measurement includes 13281 students of Isfahan University which 373 students were selected as the study sample through random sampling and sample size estimation Cochran. In order to collect data, standard questionnaire, which was made by the researcher, was used. Then, content validity of that questionnaire was verified by experts and its reliability was estimated through Cronbach Alpha 0.87. SPSS.15 was used for data analysis.Results: The results indicated that generally there is a positive relationship between the university atmosphere and students' educational motivation. Moreover, in terms of evaluating the relationship between university atmosphere indexes and students' educational motivation, the results demonstrated that there is a direct relationship between each component of university atmosphere (Contact quality, cooperation quality, student-centeredness, emotional relations) and students' educational motivation.Conclusion: Based on the findings of this study, the higher the level of university-related Contact quality, cooperative quality, student-centeredness, and emotional one, the higher the level of students' motivation will be.