The present study was conducted to investigate the effects of co-culturing of Western white shrimp, Litopenaeus vannamei and grey mullet, Mugil cephalus on diversity and incidence of vibrio bacteria from shrimp ponds during 4 months experiment. 9 earthen ponds (600 m2) as three treatments with three replicates were established in Gwadar area (located in the coastal area of Oman Sea, southeastern of Iran). The shrimp post-larvae (PL) 0.007±0.001 g in weight were stocked at density of 20 post-larvae/m2 in each experimental pond. After 40 days shrimp stocking, mullets with size of 20-50 cm were stocked at densities of 0 (control group), 2 (Treatment 1) and 4 (Treatment 2) fish per 100 m2 of ponds respectively. For bacterial study, shrimps were captured monthly as alive and tissue samples taken from gill, Hemolymph and hepatopancreas. According to bacterial inoculation data, six bacterial species including Vibrio alginoliticus, Vibrio fluvialis, Vibrio costicula, Vibrio splendidus, Vibrio nerets, Vibrio natrigenes. Bacterial incidence percent in treatment 1 (2.46%) and 2 (3.7%) were significantly lower than control group (23.47%) (P<0.05). Also, identified vibrio species in each experimental group were: (control group): Vibrio alginoliticus, Vibrio fluvialis, Vibrio costicula, Vibrio splendidus, Vibrio nerets, Vibrio natrigenes, (Treatment 1): Vibrio alginoliticus, Vibrio fluvialis, (Treatment 2): Vibrio natrigenes. In conclusion, our results show that grey mullet as a secondary species has positive effect on control of vibrio spp. of white shrimp. Likely, this effect indirectly returns to reducing of organic matter content of sediment and improvement of water quality by mullet.