Background and Objective: The maximum aerobic capacity (VO2max) is one of the criteria that can be used to create a balance between work and worker. The assessment of individuals’ physical work capacity can be used to assign individuals in jobs proportional to their capacities in order to maintain their health and physical capacity and improve their productivity and efficiency.Materials and Methods: This cross-sectional study was performed on 120 women with the age range of 21-49 years working in hospitals located in Isfahan, Iran. The participants were evaluated through the Single Stage Treadmill Walking Test by Ebbeling. The data were collected using the General Health Questionnaire-28, Occupational Fatigue Scale, and Job Satisfaction Questionnaire. Data analysis was performed in SPSS software (version 22) using the Pearson correlation and t-test.Results: According to the results, the mean VO2max, physical working capacity, and general health were obtained as 38.89±1.85 ml/kg-1.min-1, 4.10±0.47 kcal/min, and 25.43±12.09 kcal/min, respectively. In addition, the mean scores of occupational fatigue and satisfaction were 85.87±32.35 and 57.48±13.01, respectively. The results also indicated a significant relationship between VO2max and age, weight, body mass index, occupational fatigue, and job satisfaction (P<0.05).Conclusion: As the findings indicated, occupational fatigue and satisfaction were associated with VO2max among workers. Consequently, it is recommended to not only consider the compatibility of physical factors in the workplace, but also give special attention to mental aspects, since they affect the workers’ physical domain and productivity.