Introduction: Psychological well-being is a broad concept related to good or satisfactory living conditions, a state that is determined by health, joy and success. The aim of this study was to investigate the role of Islamic lifestyle and locus of control in the prediction of psychological well-being of the staff of Qazvin Bu-Ali Hospital.Methods: To do so, a regression-based descriptive study was conducted on 120 individuals from all staff categories (clinical, administrative and service personnel) working in the public Bu-Ali hospital of Qazvin using stratified random sampling method and were tested in terms of Islamic lifestyle, locus of control and psychological well-being. Data were analyzed by calculating the Pearson correlation coefficient and the multiple linear regressions.Findings: The results showed that there was a significant positive correlation between Islamic lifestyle (r=0.29) and internal locus of control (r=0.24) with psychological well-being. In addition, the regression analysis indicated that among the components of Islamic lifestyle and locus of control, the components of beliefs, family, financial and health with b=0.22, b =0.21, b =0.28, b =0.24 respectively and the internal locus of control with b =0.21 can predict the psychological well-being. In sum, the components of Islamic lifestyle and internal locus of control predict up to 15% of the variable variance of psychological well-being.Conclusion: Considering the role and importance of Islamic lifestyle and locus of control in predicting psychological well-being, it is needed to pay more attention to the application of Islamic lifestyle and locus of control in promoting psychological well-being.