Purpose: The aim of the present study was to investigate the biological sequence in articles of Iranian researchers on Information Science indexed in Web of Science untill 2015. Methodology: This quantitative study was done using a content analysis approach. The research population of the study consisted of all articles published by Iranian researchers on Information Science, which had been indexed in web of science till 2017. The data collection instrument included the thematic categorizing list (Zarei, 2014), which classified the issues in Knowledge and Information Science into 14 main subject categories, and 110 sub-categories. To analyze the content of the articles and to analyze the data, WordStat 7. 0, Excel 2013 and SPSS 20 were used. Findings: As the findings showed, among the main categories of Knowledge and Information Science in the mentioned articles, general themes in knowledge and information science, knowledge and information Science education, methodology, libraries and information centers, users’ studies, storage and retrieval of information, library and ICT, publishing and distribution industry, ethical and legal issues, and information management were in proper conditions. Conclusion: Generally speaking, the status of thematic sequence in the biological process of the reviewed articles on Information and Knowledge Science was in an acceptable level. It shows the appropriate and scientific thematic sequence of Iranian researchers in the field of Knowledge and Information Science. Biological sequence of the themes is a decisive factor in the authority of sovereignty and survival.