Fatigue as a complex phenomenon, caused by peripheral muscle weakness or by a failure to initiate or sustain voluntarily, drives to the muscle by CNS. During a soccer match, players exercised at a relatively high intensity. It is therefore not surprising that fatigue occurs, causing performance to fall off in later stages of the match in the second half Since decision-making as well as technical and tactical aspects, are important elements in soccer, fatigue towards the end of the match could cause deterioration in mental and physical performance. The availability of choline, the precursor of neuro transmitter of acetylcholine in the diet, is sufficient to provide body needs under normal condition. Preliminary evidence indicates that depletion of choline may limit performance, while oral supplementation may delay fatigue during prolonged efforts. A double-blind design was used to determine the relationship between plasma choline and acetylchline, and fatigue during soccer-specific intermittent running protocol. Thirty adult male soccer players were divided into five groups randomly; placebo group and experimental group who ingested flavored water, glucose, BCAAS, lecithin and hydroalcoholic extract of sage 2.5h before and during soccer-specific intermittent running protocol. The peripheral choline and acetylcholine levels were determined via venous blood samples collected 2.5 hours before, half-time and immediately after soccer-specific intermittent running protocol. Subjects also completed the POMS, RPE, and measures exercise time to fatigue. Based on our observations that fatigue during soccer-specific intermittent protocol or towards the end of the soccer match in adult male soccer players is associated with a reduction in plasma choline and acetylcholine, our hypothesis is that a high ratio of choline and acetycholine levels in plasma prevents peripheral fatigue and subsequently enhances performance in soccer matches.