Objective: In this study of in vitro maturation, resumption of meiosis and development of immature mouse oocytes with MEHP were evaluated.
Material and Methods: Immature oocytes recovered from NMRI mouse strain (4-6 weeks) in 4 different experimental groups and control groups. Control group: 198 immature oocytes matured in MEM-α medium contain FCS5%, rFSH 100 mIU/ml hCG7.5 IU/ml sham's group: 203 immature oocytes were matured in MEM-a medium containing FCS 5%, hCG 7.5 IU/ml, rFSH 100mIU/ml and DMSO 100µm. Group 1: 201 immature oocytes were matured in MEM- α medium containing FCS5%, hCG 7.5 IU/ml rFSH 100mIU/ml and 50 MEHP5. Group 2: 209 immature oocytes were matured in MEM- α medium containing FCS5%, hCG 7.5 IU, rFSH 100mIU/ml and 100 µm MEHP. Group 3: 203 immature oocytes were matured in MEM- α medium containing FCS5%, hCG 7.5 IU/ml rFSH 100mIU/ml and 200µm MEHP. Group 4: 204 immature oocytes were matured in MEM- α medium containing FCS5% , hCG 7.5 IU/ml rFSH 100mIU/ml and 400 µm MEHP. Fertilization and embryo development were done in T6 medium.
Results: The resumption of meiosis in experimental groups was 75%, 63%, 60%, 56% spectively. The methaphase II in experimental groups was 53%, 49%, 42%, 37%. Maturation was significant compared with a control group (86%, 90% resumption of meiosis) and 49%, 75%. Embryo formation in the experimental groups were 60%, 57%, 44%, 28% decreased in compared with control group (74%, 61%).
Conclusion: The results of this study showed that MEHP had negative effect on resumption of meiosis and in vitro maturation and development of immature mouse oocytes.