EFFECTS OF PARAOXON ON THE GROWTH OF CULTURED RAT HIPPOCAMPAL NEURONS IN NEUROBASAL/B27 MEDIUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YOUSEFPOUR M.; BAHRAMI F.; NOURIAN Z.; KHOUSHBATEN A.; ASGARI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:Part 1 Page(s): 29-35

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

603

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF PARAOXON ON THE GROWTH OF CULTURED RAT HIPPOCAMPAL NEURONS IN NEUROBASAL/B27 MEDIUM

Author(s)

Keywords

HIPPOCAMPUSQ3

CELL CULTUREQ2

PARAOXONQ3

Abstract

Objective: The qualitative morphologic study of the effects of organophosphate (OP) agents on development of neural cells in culture is promising a primitive step for further physiologic, electrophysiologic and molecular investigation. Parathion is an OP used as an agricultural insecticide. Improper handling of this agent causes poisoning characterized by convulsive seizures which may sometimes lead to death. Since parathion has been observed to be responsible for more cases of poisoning than any other OPs insecticide, it is vitally important to investigate other mechanisms, besides cholinesterase inhibition, which can contribute to the neurotoxitcity of PARAOXON (metabolite of parathion). Materials and Methods: In the present study, hippocampal cells taken from rat's neonate were introduced into the neurobasal medium supplemented with B27 serum. The neuronal growth in the control groups and those exposed to different dose of PARAOXON were compared. Phase contrast microscope and cell staining (MAP-2, Neutralred, Trypan blue) were used to study cell morphology and viability. Statistical analysis was carried out. using one way ANOVA. Results: There was no clear morphologic differences between neurons in the control group and those exposed to10µM PARAOXON; however, deformity of the soma was clear in pellets containing higher concentration of PARAOXON. The processes (neurites) did not grow in media containing 100µM PARAOXON or more. Viability decreasd with increasing PARAOXON especially above 100µM PARAOXON.Conclusion: The present data reveales decrease of cell growth, cell swelling and neuronal death elicited by higher than 30µM concentrations of PARAOXON.

Cites

No record.

References

No record.

Cite

APA: Copy

YOUSEFPOUR, M., BAHRAMI, F., NOURIAN, Z., KHOUSHBATEN, A., & ASGARI, A.. (2005). EFFECTS OF PARAOXON ON THE GROWTH OF CULTURED RAT HIPPOCAMPAL NEURONS IN NEUROBASAL/B27 MEDIUM. KOWSAR MEDICAL JOURNAL, 10(Part 1), 29-35. SID. https://sid.ir/paper/32829/en

Vancouver: Copy

YOUSEFPOUR M., BAHRAMI F., NOURIAN Z., KHOUSHBATEN A., ASGARI A.. EFFECTS OF PARAOXON ON THE GROWTH OF CULTURED RAT HIPPOCAMPAL NEURONS IN NEUROBASAL/B27 MEDIUM. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(Part 1):29-35. Available from: https://sid.ir/paper/32829/en

IEEE: Copy

M. YOUSEFPOUR, F. BAHRAMI, Z. NOURIAN, A. KHOUSHBATEN, and A. ASGARI, “EFFECTS OF PARAOXON ON THE GROWTH OF CULTURED RAT HIPPOCAMPAL NEURONS IN NEUROBASAL/B27 MEDIUM,” KOWSAR MEDICAL JOURNAL, vol. 10, no. Part 1, pp. 29–35, 2005, [Online]. Available: https://sid.ir/paper/32829/en

Related Journal Papers